对Yarrowia lipolytica进行多重修改,以通过模块化代谢工程从糖醇中增强红醇生物合成
Liang-Gang Huang1,2, Bo-Wen Xiao1,2, Wen-Jia Wang1,2
1National and Local Joint Engineering Research Center for Biomanufacturing of Choral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.
Bioprocess and biosystems engineering
|July 19, 2023
概括
研究人员对Yarrowia lipolytica进行了改造,从葡萄糖中更便宜的替代品甘油中产生高位的红醇. 这种新品种实现了创纪录的发酵效率,为可持续的化学生产铺平了道路.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 工业微生物学 工业微生物学
背景情况:
- 红醇是一种有价值的聚醇,具有作为平台化学前体的潜力.
- 葡萄糖成本上升需要替代原料,如生物生产的甘油.
- 雅罗维亚 (Yarrowia lipolytica) 是微生物合成的一个有希望的宿主.
研究的目的:
- 从糖醇中设计Yarrowia lipolytica菌株,以有效地从糖醇中产生红醇.
- 开发一种新的多模块组合策略,用于增强合成流量.
- 为了实现高滴度的红醇发酵,提高产量和生产力.
主要方法:
- 使用URA3反选择阻止退化和竞争路径.
- 通过多基因表达缩短红醇生物合成途径.
- 整合糖醇输送器ScFPS1以增强糖醇的吸收.
主要成果:
- 在5L生物反应器中,工程菌株ERY8产生了176.66g/L的红醇.
- 实现了0.631g/g的高产量和1.23g/L/h的生产率.
- 证明了迄今为止为红醇的最高发酵生产效率.
结论:
- 开发的多模块组合策略对于工程Yarrowia lipolytica是有效的.
- 糖醇是工业红醇生产的可行且具有成本效益的原料.
- 这项研究促进了平台化学品的可持续生物生产.
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