相关实验视频

Updated: Jul 23, 2025

Analysis of Histone Antibody Specificity with Peptide Microarrays

Analysis of Histone Antibody Specificity with Peptide Microarrays

Published on: August 1, 2017

通过Rpd3S进行核体脱的不同方式

Haipeng Guan^1,2, Pei Wang^1,2, Pei Zhang^1,2

¹State Key Laboratory of Molecular Oncology, MOE Key Laboratory of Protein Sciences, SXMU-Tsinghua Collaborative Innovation Center for Frontier Medicine, School of Medicine, Tsinghua University, Beijing, China.

|July 19, 2023

概括

Rpd3S复合体使用独特的结构来识别像H3K36me3这样的基因标记,指导基因调节. 这种表观遗传机制表现出复杂的,动态的核酶参与精确的脱乙烯化.

科学领域:

表观遗传学
分子生物学
结构生物学

背景情况:

动态基因组修饰对于基因调节至关重要.
该Rpd3小 (Rpd3S) 复合体参与由H3K36me3标记指导的基因组脱乙烯化.

研究的目的:

阐明Rpd3S功能的结构基础.
了解Rpd3S核细胞识别和脱乙的机制.

主要方法:

用冷电子显微镜测定Rpd3S的结构.
用于研究核细胞结合和脱活性的生物化学测试.

主要成果:

确定了Saccharomyces cerevisiae Rpd3S在自由状态和H3K36me3核细胞结合状态中的结构.
揭示了Rpd3S的独特不对称结构,其中包括Eaf3-Rco1异构体,Rpd3和Sin3.
证明了对H3K36me3,核体DNA和链接器DNA进行H4脱乙的多价值识别.
确定了一种涉及H3K4和H3K36me3的替代催化模式,用于特定的H3脱乙烯化.

结论:

Rpd3S采用了动态和多样化的核细胞参与模式.
通过Rpd3S进行甲基化引导的脱乙化突显了表观遗传调节的复杂性.
多个子单位的Rpd3S机器在转录和其他细胞过程中发挥着关键作用.

更多相关视频

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

相关概念视频

Spreading of Chromatin Modifications

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...

Histone Modification

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...

Position-effect Variegation

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.

Heterochromatin

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...

Histone Variants at the Centromere

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...

Chromatin Modification in iPS Cells

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...