通过NEAT1介导的miR-150-5p下调调节,调节了OA胆固醇细胞中的b-catenin表达
Ioanna Papathanasiou1,2, Charalampos Balis1, Dimitrios Destounis1
1Faculty of Medicine, Laboratory of Cytogenetics and Molecular Genetics, University of Thessaly, Biopolis, 41500, Larissa, Greece.
Functional & integrative genomics
|July 19, 2023
概括
这项研究揭示了长非编码RNA NEAT1和microRNA miR-150-5p通过调节β-catenin表达来促进骨关节炎 (OA),从而影响软骨退化. 了解这个NEAT1/miR-150-5p轴可以为OA的发病过程提供新的见解.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 生物化学 生物化学
背景情况:
- 骨关节炎 (OA) 是一种退行性关节疾病,其特征是软骨的破坏.
- 微RNAs (miRNAs) 和长非编码RNAs (lncRNAs) 在OA病变发生过程中的作用越来越被认可.
- 特定的分子相互作用,如涉及miR-150-5p和lncRNAs的相互作用,需要在OA冠状细胞中进一步阐明.
研究的目的:
- 为了研究miR-150-5p在骨关节炎 (OA) 冠状细胞中的功能.
- 探索lncRNAs,特别是NEAT1在调节miR-150-5p表达中的调节作用.
- 为了阐明与OA相关的软骨降解中的NEAT1/miR-150-5p/β-catenin通路.
主要方法:
- 使用TargetScan,StarBase,DIANA-LncBase和Open Targets等数据库进行生物信息分析,以预测相互作用和目标.
- 使用STRING构建一个蛋白质-蛋白质相互作用网络.
- 基因本体学 (GO) 和途径分析通过Enrichr.
- 分析RNA测序数据以识别差异表达的lncRNAs.
- 定量PCR (qPCR) 和西部斑点测试用于测量基因和蛋白质表达.
- 在体外实验包括lncRNA淘汰和细胞转染.
主要成果:
- 生物信息学预测β-catenin作为一个目标的miR-150-5p.
- 在OA冠状细胞中,miR-150-5p的过度表达减少了β-catenin,MMP-13和ADAMTS-5的表达.
- NEAT1淘汰赛增加了OA胆固醇细胞中的miR-150-5p表达,这表明NEAT1对miR-150-5p进行负面调节.
- 抑制miR-150-5p可以逆转NEAT1沉默对β-catenin表达的影响.
结论:
- 这项研究确定了OA冠状细胞中NEAT1和miR-150-5p之间的功能相互作用.
- 这种NEAT1/miR-150-5p轴似乎通过调节β-catenin.p在OA进展中起着代谢作用.
- 研究结果表明NEAT1和miR-150-5p是骨关节炎的潜在治疗点.
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