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相关概念视频

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

828
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

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The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
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化学量子阵列:用于质谱成像的定量工具.

Sylwia A Stopka1,2, Daniela Ruiz1,3, Gerard Baquer1

  • 1Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, United States.

Analytical chemistry
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概括
此摘要是机器生成的。

化学QuantArray增强了矩阵辅助激光脱离离电离质谱成像 (MALDI-MSI) 用于代谢物量化. 这种新的方法通过直接在组织微阵列上创建校准曲线来提高临床应用的精度和准确性.

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科学领域:

  • 分析化学 分析化学
  • 生物医学成像技术 生物医学成像技术
  • 代谢学 代谢学 代谢学

背景情况:

  • 矩阵辅助激光吸附离子化质谱成像 (MALDI-MSI) 提供空间代谢物检测,但需要更好的临床翻译.
  • 目前的MALDI-MSI方法在临床应用中的吞吐量,精度和准确性方面面临挑战.

研究的目的:

  • 开发一种使用MALDI-MSI进行精确和准确的代谢物量化的改进方法.
  • 提高MALDI-MSI用于分析组织样本的临床可行性.

主要方法:

  • 开发了"化学量子阵列",一种凝组织微阵列 (TMA) 模具,以同位素标记的代谢物标准的连续稀释.
  • 将TMA冷切割到组织均质上以生成校准曲线.
  • 实施自动删除像素并研究强度正常化策略,包括内部标准.

主要成果:

  • 化学QuantArray使内源代谢产物的广泛动态范围量化成为可能.
  • 该技术可以将MALDI幻灯片上的校准标准空间减少80%左右.
  • 通过像素去除和规范化实现精度 (<20% RSD) 和准确性 (<20% DEV).
  • 在单个幻灯片上成功量化了14个临床瘤样本中的多重精氨酸代谢物.

结论:

  • 化学QuantArray显著提高了MALDI-MSI代谢物量化的分析性能.
  • 这种方法提高了临床和翻译研究的实际可行性.
  • 该技术为复杂的生物样本中的空间代谢学提供了更有效和可靠的方法.