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相关概念视频

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

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Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
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Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

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Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
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Master Transcription Regulators02:23

Master Transcription Regulators

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

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De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
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The Bone Matrix01:18

The Bone Matrix

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Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in...
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Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
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长非编码RNACASC2调节骨质细胞的基质矿化.

Jaime Freitas1,2, Sara Reis Moura1,2,3, Mário Adolfo Barbosa1,2

  • 1i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.

Frontiers in bioengineering and biotechnology
|July 20, 2023
PubMed
概括

长的非编码RNACASC2通过影响COMP和BSP的骨质分化来调节骨矿化. 准CASC2为骨矿化障碍提供了潜在的治疗策略.

关键词:
骨头 骨头 骨头 骨头细胞分化的细胞分化.细胞外矩阵是细胞外矩阵.长时间的非编码转录.介酶干细胞/介酶干细胞.

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物化学 生物化学

背景情况:

  • 长非编码RNAs (lncRNAs) 是具有新兴治疗潜力的关键基因调节者.
  • LncRNA放松调节与骨质疏松症等骨疾病有关.
  • 在骨生理学和病理学中 lncRNAs 的精确作用需要进一步研究.

研究的目的:

  • 研究 lncRNA CASC2 在骨质分化和矿化中的作用.
  • 探索CASC2作为骨健康的潜在治疗点.

主要方法:

  • 研究了在人类骨髓衍生中介质干细胞/骨干细胞 (hMSCs) 的骨质基因分化过程中的CASC2表达.
  • 利用小干扰RNA对抗CASC2 (siCASC2) 进行基因淘汰.
  • 进行蛋白质组分析并评估骨质原生标志物 (BSP,RUNX2,OPG,ALP) 和矿化 (沉积物).

主要成果:

  • 在骨质分化过程中,CASC2表达减少.
  • siCASC2增加了晚期的骨质原生标志物骨蛋白 (BSP),并促进了矿化.
  • 在转录和蛋白质水平上,CASC2敲击调节了软骨寡合基质基质蛋白 (COMP).
  • 抑制COMP导致骨质母细胞矿化和BSP表达受损.

结论:

  • lncRNA CASC2通过COMP和BSP调节hMSC的晚期骨质生成分化和矿化.
  • 准 lncRNA CASC2 是调节骨矿化的一个潜在策略.