鉴定急性过敏和ST段升高心肌梗塞共享的生物过程,免疫细胞格局和枢纽基因
Zekun Peng1, Hong Chen1, Miao Wang1,2
1State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Frontiers in pharmacology
|July 20, 2023
概括
过敏反应和ST段升高心肌梗塞 (STEMI) 具有相同的炎症途径和免疫细胞概况. 确定了关键的诊断生物标志物DUSP1和CLEC4D,为向治疗提供了潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 心脏病学 心脏病学
- 基因组学就是基因组学.
背景情况:
- 过敏反应患者面临ST段升高心肌梗塞 (STEMI) 的风险增加,但潜在的病理联系尚不清楚.
- 研究共享的生物过程,免疫细胞和基因表达模式对于理解这种关联至关重要.
研究的目的:
- 探索共享的生物过程,免疫效应细胞和过敏和STEMI之间的枢纽基因.
- 为了确定潜在的诊断生物标志物和治疗目标的STEMI在过敏症患者.
主要方法:
- 利用了对过敏反应和STEMI患者和健康对照者的基因表达综合数据集.
- 进行了差异基因表达分析,丰富分析,用于免疫细胞分析的CIBERSORT,并确定了常见的差异表达基因 (DEG).
- 在外部数据集和小鼠模型中验证了枢纽基因,通过后勤回归评估诊断效率.
主要成果:
- 鉴定了265个与过敏相关的DEGs (免疫-炎症反应) 和237个与STEMI相关的DEGs (先天性免疫反应,骨髓白细胞激活).
- 在这两种条件下发现M0巨细胞和树突细胞的增加,CD4+原始T细胞和CD8+T细胞的减少.
- 确定了六个共享的枢纽基因,其中五个 (IL1R2,FOS,MMP9,DUSP1,CLEC4D) 在外部数据集中升级,并在小鼠模型中得到证实;DUSP1和CLEC4D显示出诊断潜力.
结论:
- 过敏反应和STEMI具有共同的炎症和防御反应途径.
- 特定的免疫细胞 (巨细胞,树突细胞,T细胞) 都与这两种疾病有关.
- 枢纽基因DUSP1和CLEC4D代表了在过敏相关的STEMI中诊断,预后和治疗策略的有希望的候选人.
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