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开发HPLC-UV方法,同时确定与皮质类固醇联合使用的免疫疗法

Nessreen S Abdelhamid¹, Nehal F Farid¹, Basma H Anwar¹

¹Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St., 62514 Beni-Suef, Egypt.

Journal of chromatographic science

|July 20, 2023

概括

一种新的,环保的HPLC方法准确地测量了人体血中的普雷尼索隆 (PDS) 和埃索梅普拉 (ESM). 这种经过验证的技术确保了这些重要药物的安全同时使用.

科学领域:

分析化学分析化学
药品分析药品分析

背景情况:

普雷迪尼索隆 (PDS) 用于自身免疫性疾病,癌症和COVID-19相关的呼吸系统问题.
与埃索梅普拉 (ESM) 联合使用是必要的,以减轻PDS诱导的胃肠道.
在生物矩阵中精确量化PDS和ESM对于治疗监测至关重要.

研究的目的:

开发和验证一种灵敏,环保的高性能液体染色学 (HPLC) 方法.
量化它们的二元组合和的人体血中的普雷尼索隆 (PDS) 和埃索梅普拉 (ESM).
评估开发的分析方法的绿色性.

主要方法:

使用C8柱与渐变化和在290nm的紫外线检测用于药物分离.
采用咖啡因作为内部标准,以纠正血样本变化.
使用绿色分析程序指数,分析绿色度量和分析生态尺度评估方法的绿色度.

主要成果:

实现了血,咖啡因,ESM和PDS的分离,其保留时间 (tR) 分别为1.4,3.5,6.3和7.3分钟.
开发的HPLC方法显示了令人满意的绿色特性.
验证参数符合美国FDA的建议,证实适合生物分析.

结论:

开发的HPLC方法对于分析PDS和ESM是敏感,快速和环境可持续的.
这种经过验证的方法支持PDS和ESM组合的安全有效的治疗使用.
该研究强调了绿色分析化学在制药分析中的重要性.

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