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磁珠球形核酸微结构可用于可靠的DNA保存和重复的DNA读取

Peng Shen¹, Xiaojun Qu², Qinyu Ge¹

¹State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China.

ACS synthetic biology

|July 20, 2023

概括

这项研究引入了磁珠球形核酸 (MB-SNA) 用于可靠的DNA数据存储. MB-SNA确保了长期保存和可重复读取,克服了DNA存储技术的关键挑战.

科学领域:

生物技术是生物技术.
材料科学材料科学材料科学
数据存储数据存储数据存储

背景情况:

对于数据存储,DNA提供了高密度,可持续性和寿命.
当前的DNA存储在保存可靠性和读取可重复性方面面临着挑战.
编码,自动化和测序方面的进展正在进行中.

研究的目的:

开发一个具有成本效益的平台,可靠的DNA保存和重复阅读.
构建和评估用于数据存储的磁珠球形核酸 (MB-SNA) 复合微结构.

主要方法:

用一个silica@γ-Fe2O3@silica微珠芯和一个dsDNA外制造MB-SNA.
双链DNA (dsDNA) 的高密度包装用于高容量存储.
加速衰老试验和聚合酶链反应 (PCR) -磁性分离用于保存和读取评估.

主要成果:

在 -18°C和50%的RH下保存了023万年后,MB-SNA证明了数据的可读性.
经过10个PCR周期后,MB-SNA显示dsDNA保留率为93%,测序精度超过98%.
只有两个MB-SNA微结构可以实现准确的读数.

结论:

MB-SNA为可靠的DNA数据存储提供了强大且具有成本效益的解决方案.
该MB-SNA平台显著提高了DNA的保存,并使可重复的数据访问.
这项技术解决了DNA数据存储的实际部署中的关键障碍.

关键词:

储存 DNA DNA 的储存.磁性珠子磁性珠子保护保护保存保存可重复阅读的可重复阅读球形核酸的核酸是一种球形核酸.

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