酸通过与Arabidopsis中的AMMONIUM TRANSPORTER 1;1相互作用来调节氨的吸收
Hongwei Cao1, Qingyun Liu1, Xiao Liu1
1State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Plant physiology
|July 20, 2023
概括
酸 (PA) 通过与转运器1 (AMT1) 相互作用来调节植物中 (NH4+) 的吸收. 这种基于脂质的机制控制NH4+运输和植物适应气条件.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 氨 (NH4+) 对于植物氨基酸生物合成至关重要.
- 氨运输器 (AMT) 规则平衡NH4+吸收以适应的可用性.
- 脂酶D (PLD) 衍生的酸 (PA) 是植物中的信号脂质.
研究的目的:
- 为了研究酸 (PA) 在调节 (NH4+) 吸收中所起的作用,转运器1:1 (AMT1;1) 在Arabidopsis.
- 阐明PA-AMT1;1相互作用的分子机制及其对NH4+运输和植物生长的影响.
主要方法:
- 对Arabidopsis thaliana中的pldα1 pldδ-knockout突变物的分析.
- 生物化学试验以确定PA与AMT1的结合1;1.1.
- 在体外和体外测定氨运输活性.
- AMT1;1-GFP局部化和内细胞形成的同焦显微镜.
主要成果:
- 在pldα1 pldδ突变体中降低的PA水平在缺乏下增强了幼苗的生长,在NH4+压力下抑制了根的生长,这表明NH4+积累发生了改变.
- PA直接与AMT1;1结合,抑制其运输活动.
- 在PA结合部位 (R487G) 的AMT1;1突变取消了PA介导的抑制,并增强了NH4+运输.
- PLD缺陷或AMT1;1突变抑制了AMT1;1内细胞分裂,在野生类型中被PA拯救,但在R487G突变中没有.
结论:
- 酸 (PA) 作为植物氨 (NH4+) 运输的负调节剂,直接与氨运输器1 (AMT1) 相互作用.
- PA抑制AMT1;1活动并促进其内细胞分裂,为NH4+吸收提供一个关闭机制.
- 这项研究揭示了一种新的基于脂质的调节途径,控制了植物中的营养物质运输,并突出了脂质在传送器功能中的更广泛作用.
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