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相关概念视频

Oligosaccharide Assembly01:24

Oligosaccharide Assembly

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Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
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Proteoglycans01:05

Proteoglycans

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Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
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Protein Glycosylation01:25

Protein Glycosylation

7.0K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
7.0K
Glycocalyx and its Functions01:14

Glycocalyx and its Functions

4.2K
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
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Matrix Proteoglycans and Glycoproteins01:21

Matrix Proteoglycans and Glycoproteins

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Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...
4.0K
Glycosaminoglycans01:23

Glycosaminoglycans

4.9K
Glycosaminoglycans (GAGs), also known as mucopolysaccharides, are long and linear polymers comprising of specific repeating disaccharides - the amino sugar that can be N-acetylglucosamine or N-acetylgalactosamine, and a uronic acid that is usually glucuronic acid or iduronic acid.
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...
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基于ROMP的高亲和度的甘油聚合物对曼诺斯结合性莱克具有很高的亲和度.

Clément Gonnot1, Mathieu Scalabrini2, Benoit Roubinet3

  • 1Institut des Molécules et Matériaux du Mans (IMMM), UMR 6283 CNRS - Le Mans Université, Avenue Olivier Messiaen, 72085 Le Mans Cedex 9 France.

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概括

新型甘油聚合物是使用环开放转化聚合和后聚合修饰合成的. 这些先进的材料表现出强大的多价值抑制碳水化合物-乳氨酸相互作用,显示治疗应用的希望.

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科学领域:

  • 聚合物化学 聚合物化学
  • 碳水化合物化学 碳水化合物化学
  • 生物材料科学 生物材料科学

背景情况:

  • 乳清素是能结合碳水化合物的蛋白质,在生物过程中起作用.
  • 调节莱克活性对于理解和治疗莱克介导疾病至关重要.
  • 多价值物相互作用可以显著增强结合亲和力和生物活性.

研究的目的:

  • 为了合成具有受控架构的明确定义的甘油聚合物.
  • 研究这些甘油聚合物的潜力,以抑制碳水化合物-莱克相互作用.
  • 探索多价值物对结合抑制的影响.

主要方法:

  • 诺伯尼尔亚兹拉克的环开转化聚合 (ROMP) 形成聚合物骨干.
  • 通过点击氨解进行后聚合修饰 (PPM) 来附加各种糖化物.
  • 对使用合成甘油聚合物的莱克组进行结合抑制的评估.

主要成果:

  • 成功合成了具有受控分子量的多 (norbornenyl azlactone) 化合物.
  • 与基化曼诺化物功能化的甘油聚合物显示出强烈的结合抑制 (亚纳米分子IC50s).
  • 由于多价值效应,聚合物与单价值类似物相比显著增强了抑制活性.

结论:

  • 糖功能化多 (norbornenyl azlactone) 是研究多价值碳水化合物-乳素相互作用的有效工具.
  • 这些甘油聚合物对向莱克介导的细菌和病毒与宿主细胞结合的应用具有前景.
  • 该研究强调了聚合物设计和多价值呈现在调节生物相互作用中的力量.