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CircKDM5B海绵miR-128通过调节体壁垒功能来调节猪胚胎细胞的发育

Di Gao^1,2, Xin Wang³, Ye-Lian Yan³

¹Shenzhen Key Laboratory of Fertility Regulation, Center of Assisted Reproduction and Embryology, The University of Hong Kong Shenzhen Hospital, Shenzhen, China.

Molecular human reproduction

|July 20, 2023

概括

循环RNAs (circRNAs) 调节基因表达. 在猪中,circKDM5B对早期胚胎发育至关重要,通过海绵化miR-128影响细胞数量和紧密结合组件.

科学领域:

* 发育生物学发育生物学
* 分子生物学 * 分子生物学
* 遗传学遗传学是一门学科

背景情况:

*圆形RNAs (circRNAs) 是哺乳动物基因表达的关键调节者.
*它们在哺乳动物植入前胚胎发育中的特定作用尚不清楚.
*了解这些作用对于生殖生物学和发育研究至关重要.

研究的目的:

* 调查猪早期胚胎发育中的circRNAs的功能.
* 确定参与调节关键发育过程的特定circRNAs.
*阐明胚胎中循环RNA介导调节的分子机制.

主要方法:

*使用集成测序数据分析对猪胚胎中的circRNAs进行查.
* 功能性研究涉及circRNA淘汰和microRNA抑制剂注射.
*评估胚芽细胞的形成,细胞数和紧密结合的完整性.

主要成果:

*鉴定了circKDM5B,它来自基因组脱甲基酶KDM5B,在猪胚胎中表达很高.
*circKDM5B knockdown显著损害了胚胎细胞形成,减少了细胞数量,并破坏了紧密结合组件.
*circKDM5B作为miR-128的海绵,在注射抑制剂时挽救发育缺陷.

结论:

* circKDM5B在猪早期胚胎发育中起着至关重要的作用.
* 它作为miR-128的分子海绵,调节基因表达.
*这种调节对于细胞增殖和通过紧密结合建立上皮质完整性至关重要.

关键词:

胚胎细胞的发育过程.圆形的RNAs是圆形的RNAs.胚胎发育过程中的胚胎发育.猪类动物猪类动物紧密的交叉路口紧密的连接皮肤上的托菲克托多德.

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