类似MdHB7的药物通过促进自活动和Na+排泄物来积极调节果盐耐受性
Jie Yang1, Lina Qiu1, Quanlin Mei1
1State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
The Plant journal : for cell and molecular biology
|July 20, 2023
概括
果盐耐受性涉及与自相关的基因MdATG18a和阴离子/Ca2+交换器MdCCX1.1. 转录因子MdHB7-like调节两者,通过不同的途径增强植物对盐应激的抵抗力.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 盐应激对作物产量和分布产生负面影响.
- 自对于植物的非生物应激耐受性至关重要,但盐诱导的机制尚不清楚.
- 阴离子/Ca2+交换剂影响植物的盐分耐受性,但它们的具体作用需要阐明.
研究的目的:
- 研究与自相关的基因MdATG18a在果盐耐受性中的作用.
- 阐明通过转录因子MdHB7-like调节盐应激反应的调节机制.
- 确定MdHB7-like在调节自和离子运输中对盐分耐受性的参与.
主要方法:
- 在果中基因过度表达.
- 自抑制试验. 自抑制试验.
- 酵母-一混合测定促进体结合.
- 转录和遗传分析.
- 对转基因酵母中Na+排放的分析.
主要成果:
- 过度表达MdATG18a增强了盐分耐受性,增加了自,プロ林和抗氧化酶活性,同时减少了Na+积累.
- 类似MdHB7的药物直接与MdATG18a促进体结合,上调其表达并增强盐诱导的自.
- 类似MdHB7的药物也直接促进MdCCX1的表达,增强Na+流量,而不依赖于自.
结论:
- 类似MdHB7的药物通过两个不同的模块调解果盐耐受性:类似MdHB7的MdATG18a (自依赖) 和类似MdHB7的MdCCX1 (自独立).
- 这些发现提供了关于植物盐诱导的自调节和耐压机制的见解.
- 这项研究为培育耐盐作物提供了基础.
更多相关视频
07:38Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane
Published on: March 30, 2015
9.3K
09:29Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
Published on: March 5, 2019
5.8K
相关概念视频
Responses to Salt Stress
13.2K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.2K
Factors Influencing Microbial Growth: Osmolarity
44
Osmolarity is the measure of solute concentration in a solution. It plays a critical role in determining water availability for organisms. Water moves across semipermeable membranes through osmosis, flowing from regions of lower solute concentration (more dilute) to regions of higher solute concentration (more concentrated).In high-solute environments, microbial cells lose water, leading to dehydration and inhibited growth. The extent to which water is available to microbes in such environments...
44
Autophagy
4.4K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.4K
Delivery Pathways to the Lysosome
6.6K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.6K
