通过在IPEC-J2细胞中结合VIM,NLRP9参与抗病毒先天免疫
Ge Qin1, Xiang Yu2, Yuanjie Zhao2
1School of Animal Science and Technology, Hainan University, Hainan, Haikou, 570228, PR China; College of Animal Science, Fujian Agriculture and Forestry University, Fujian, Fuzhou, 350002, PR China.
Developmental and comparative immunology
|July 20, 2023
概括
核酸结合性寡合化域 (NOD) 类受体与含有胺域 (PYD) 的蛋白9 (NLRP9) 在猪肠表达,并在抗病毒天生的免疫力中发挥作用. NLRP9与维门丁相互作用,调节粘膜免疫力.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 核酸结合性寡合化域 (NOD) 类受体与含有胺域 (PYD) 的蛋白9 (NLRP9) 最初被认为是生殖系统的专用.
- 新出现的证据表明NLRP9参与传染病和炎症性疾病.
研究的目的:
- 为了研究猪组织和IPEC-J2细胞中的NLRP9表达.
- 阐明NLRP9在抗病毒天生的免疫力中的作用及其与Vimentin的相互作用.
主要方法:
- 在小猪组织和IPEC-J2细胞中对NLRP9mRNA和蛋白质表达的定量分析.
- 用LPS和多I:C刺激IPEC-J2细胞以评估NLRP9调控.
- 基因干扰,以评估NLRP9对抗病毒基因表达的影响.
- 同免疫沉以确认NLRP9-Vimentin的相互作用.
主要成果:
- 在猪肠和IPEC-J2细胞中观察到高NLRP9表达.
- LPS和多I:C显著提高了NLRP9蛋白水平.
- 聚I:C诱导的抗病毒基因 (NF-κB,IRF3,IRF7,ISG15,ISG56,OAS1,IFNB1),在NLRP9干扰时被下调.
- 发现NLRP9与Vimentin相互作用.
结论:
- NLRP9在猪肠表达,在抗病毒天生的免疫力中起着至关重要的作用.
- NLRP9可能通过与维门结合而起作用,有助于调节粘膜免疫力.
- 这些发现为猪肠道免疫的分子机制提供了洞察力.
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