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在IGF2BP3-EGFR-AKT轴促进乳腺癌MDA-MB-231细胞生长

Xintao Jing¹, Cong Han², Qian Li³

¹Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University School of Health Science Center, Xi'an 710061, Shaanxi, China; Key Laboratory of Environmentally and Genetically Associated Diseases, Xi'an Jiaotong University School of Health Science Center, Xi'an 710061, Shaanxi, China.

Biochimica et biophysica acta. Molecular cell research

|July 20, 2023

概括

胰岛素样生长因子2 mRNA结合蛋白3 (IGF2BP3) 是乳腺癌的关键调节者. 这项研究显示,IGF2BP3增强了EGFR-AKT通路,促进了三阴性乳腺癌细胞的增殖.

科学领域:

分子瘤学分子瘤学
癌症基因组学癌症基因组学

背景情况:

胰岛素样生长因子2 mRNA结合蛋白3 (IGF2BP3) 是一种与各种癌症恶性瘤相关的新兴预后指标.
特别是在乳腺癌中,IGF2BP3的调节网络在很大程度上仍未被描述.

研究的目的:

确定IGF2BP3在乳腺癌MDA-MB-231细胞中的直接调控标.
阐明IGF2BP3在癌症进展中调节的功能机制和途径.

主要方法:

RNA免疫沉降测序 (RIP-seq) 用于识别与IGF2BP3结合的RNA.
高通量RNA测序 (RNA-seq) 用于分析基因表达变化.
功能性试验调查IGF2BP3在细胞增殖和信号通路中的作用.

主要成果:

IGF2BP3直接结合并稳定表皮生长因子受体 (EGFR) mRNA,增强EGFR的表达.
IGF2BP3通过EGFR/AKT信号轴促进MDA-MB-231细胞的增殖.
确定了丰富的途径,包括炎症反应,内质网膜应激和细胞循环调节.

结论:

在三阴性乳腺癌中,IGF2BP3通过稳定EGFR mRNA并激活EGFR/AKT通路,起到瘤基因的作用.
在促进增殖方面,IGF2BP3的作用独立于EGFR信号调制.
IGF2BP3代表了三阴性乳腺癌的潜在治疗标.

关键词:

乳腺癌乳腺癌乳腺癌欧洲农业基金会 (EGFR) 是一个基金.在 IGF2BP3BP3 中.在RIP-seq和RNA-seq中使用.

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