转录因子BnaA9.WRKY47协调了Brassica napus中的叶子衰老和再动员
Rui Cui1,2, Yingna Feng1,2, Jinliang Yao2
1National Key Laboratory of Crop Genetic Improvement, Wuhan 430070, China.
Journal of experimental botany
|July 20, 2023
概括
一个新发现的转录因子BnaA9.WRKY47通过促进叶子衰老和营养转移来增强油菜 (Brassica napus) 的气再调动,从而提高了缺乏的情况下的种子产量.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 农业科学 农业科学
背景情况:
- (N) 对植物生长至关重要,其重新调动对于适应N缺乏至关重要.
- 了解Brassica napus (油菜) 中的N转移调节对于改善作物至关重要.
研究的目的:
- 识别和描述布拉西卡纳普斯 (Brassica napus) 中移动的调节机制.
- 研究转录因子BnaA9.WRKY47在N饥饿反应中的作用及其对植物生长和产量的影响.
主要方法:
- 在N饥饿状态下在甘露中识别BnaA9.WRKY47.
- 在N缺乏条件下分析BnaA9.WRKY47-过度表达 (OE) 和淘汰 (KO) 线.
- 生物化学测试以确定植物组织中的基因激活和N含量.
主要成果:
- 过度表达BnaA9.WRKY47加速了叶子衰老,并促进了N从老叶/种子移动到年轻叶/种子.
- 在OE系中,在N缺乏下,种子产量增加,而KO突变体的产量减少.
- BnaA9.WRKY47 直接激活与衰老相关的基因 BnaC7.SGR1 和 N 载体基因 BnaA9.AAP1 和 BnaA2.NRT1.7.7 的基因.
结论:
- BnaA9.WRKY47 是一个关键的调节器,促进在N饥饿下Brassica napus中的N重新调动.
- 这种机制增强了植物对缺乏的适应性,并提高了种子产量.
- 准BnaA9.WRKY47为开发无N效的油菜品种提供了一种策略.
关键词:
布拉斯卡纳普斯 (Brassica napus) 是一种植物.N 饥饿的死亡这就是WRKYY.卡诺拉 (canola) 是一种大豆.叶子衰老 叶子衰老气重新调动气的重新调动油脂种子的强奸种子产量产生种子产量.转录因子的转录因子更多相关视频
11:33Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Published on: October 14, 2022
1.6K
10:58Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Published on: December 23, 2017
12.7K
相关概念视频
Gene Regulation During Sporulation
36
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
36
Adaptations that Reduce Water Loss
25.8K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.8K
Cell Signaling in Plants
5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K
Co-activators and Co-repressors
7.4K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.4K
Bacterial Protein Maturation
36
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
36
Transcription
147.3K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
147.3K
