在小鼠神经肌肉结节的 postsynaptic 挤出会化突触裂
Ryan J Durbin1,2, Dante J Heredia2, Thomas W Gould1,2
1Integrative Neuroscience Graduate Program, University of Nevada, Reno, Reno, Nevada 89557.
概括
神经肌肉结 (NMJ) 的细胞外pH值变化取决于刺激. 短暂的神经刺激会导致化,而长时间的刺激会导致酸化,影响神经传递.
科学领域:
- 神经科学是一个神经科学.
- 生理学 生理学 生理学
背景情况:
- 细胞外pH值通过影响发射器释放和受体活性显著影响神经传递.
- 突触裂的pH值可以由于囊泡释放或离子运输机制而酸化或化.
- 在生理条件下,哺乳动物神经肌肉结 (NMJ) 的pH动态尚未得到充分理解.
研究的目的:
- 为了研究哺乳动物NMJ的突触裂中的细胞外pH过渡.
- 确定神经传递过程中这些pH值变化背后的来源和机制.
主要方法:
- 在小鼠肌肉中利用pH敏感探针pHusion-Ex的病毒表达来监测突触裂pH.
- 采用GCaMP3来追踪 postsynaptic 细胞内 (Ca2+) 的动态.
- 操纵神经刺激参数 (频率和持续时间) 并抑制了血膜Ca2+ATPase (PMCA).
主要成果:
- 短暂的神经刺激 (5秒50赫兹) 诱导显著和长时间的化,取决于后突触Ca2+释放.
- 持续刺激 (20秒50赫兹) 导致突触裂的长时间净酸化.
- 后突触Ca2+释放与裂口化正相关,PMCA抑制降低了这种化.
结论:
- 鼠标NMJ中的胆固醇突触通常由于活动诱导的细胞内Ca2+释放而化.
- 在强烈刺激下,NMJ表现为净酸化.
- 这些双向的pH变化取决于使用情况,并为突触可塑性和潜在的治疗点提供了新的见解.
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