在废物活化泥中积累的聚酸的可视化
Ruizhe Pei1,2, Gerard Vicente-Venegas2, Agnieszka Tomaszewska-Porada2
1Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.
Environmental science & technology
|July 20, 2023
概括
聚酸酸盐 (PHAs) 在废水污泥中产生. 新的显微镜方法可视化了PHA的储存,显示出有限但强烈的细菌积累,这对于优化恢复至关重要.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 微生物生态学 微生物生态学
背景情况:
- 市政废水活性污泥可以成为生产聚酸酸盐 (PHAs) 的来源.
- 了解微生物群落中PHA储存分布是过程优化的关键.
- 现有的方法缺乏分辨率,以详细描述活化污泥流体内PHA积累的情况.
研究的目的:
- 开发和优化使用共聚焦激光扫描显微镜 (CLSM) 的选择性光染色方法.
- 评估活性污泥中PHA储存活动的分布和异质性.
- 为增强PHA生产和回收提供结构功能关系的见解.
主要方法:
- 选择性光染色技术的开发和优化.
- 应用CLSM可视化PHA颗粒在活性污泥流体内.
- 对绘制PHA分布图像的染色方法进行比较分析.
主要成果:
- 活性污泥生物质中很大,但有限的部分活跃积累了PHAs.
- PHA颗粒在微生物中和微生物之间分布异质.
- 储存PHA的细菌的细胞内PHA含量明显高于平均生物质.
- 优化的染色方法为成像PHA分布提供了高敏度,超过了以前的技术.
结论:
- 选择性染色和CLSM可视化揭示了活性污泥中PHA分布的详细模式.
- 不同质的PHA积累意味着单个细菌的储存容量比批量测量表明的更高.
- 这些发现对优化废水生物质中PHA回收策略具有重大意义.
- 先进的可视化工具为改善生物废水处理和生物聚合物生产过程提供了关键的见解.
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