外体环TGFBR2通过增强ATG5介导的保护性自促进肝细胞癌的进展
Xin Wang1,2, Feng-Lin Dong1, Ying-Qiao Wang3
1Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, 250117, China.
Cell death & disease
|July 20, 2023
概括
外体体中的循环RNAs (circRNAs) 促进肝细胞癌 (HCC) 的进展. CircTGFBR2通过circTGFBR2/miR-205-5p/ATG5通路促进自,从而增强HCC细胞对饥饿的抵抗力.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 外基因组调解细胞间通信,在瘤进展中至关重要.
- 外体内循环RNA (circRNAs) 在这种通信中起着重要的作用.
- 肝细胞癌 (HCC) 细胞在压力下表现出改变的外体体载荷.
研究的目的:
- 研究饥饿压力肝细胞外体中的circRNAs.
- 确定HCC进展中的特定circRNAs的功能和机制.
- 确定HCC的新型治疗点.
主要方法:
- RNA测序以识别差异表达的环RNAs.
- 分子测试 (RNA拉向,RIP,双露西法酶记者测试) 来阐明机制.
- 在体外和体内实验 (救援试验,瘤异种移植试验) 来验证发现.
主要成果:
- 鉴定了丰富的circTGFBR2在饥饿压力肝细胞外体中.
- 通过外体传递的 circTGFBR2 增强了 HCC 细胞对饥饿的抵抗力.
- 已证明circTGFBR2作为miR-205-5p的竞争性内源RNA,上调ATG5并促进自.
结论:
- CircTGFBR2是一种新型的瘤促进性circRNA在肝细胞外体中.
- 通过通过circTGFBR2/miR-205-5p/ATG5轴促进ATG5介导的自,CircTGFBR2增强了HCC的进展.
- 这一途径代表了HCC的潜在治疗标.
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