敏感的多色指标用于监测上腺素 in vivo
Zacharoula Kagiampaki1, Valentin Rohner1, Cedric Kiss1
1Institute of Pharmacology and Toxicology, University of Zürich, Zürich, Switzerland.
Nature methods
|July 20, 2023
概括
研究人员开发了对上腺素 (NE) 的新敏感多色指标,以可视化其在大脑中的释放. 这些新的工具改善了NE信号的体内成像,这对于理解大脑功能至关重要.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 来自G蛋白结合受体的基因编码指标对于体内神经调节器成像至关重要.
- 准确的可视化北上腺素 (NE) 释放对于理解其在神经过程中的作用至关重要.
研究的目的:
- 引入一种新型的敏感多颜色的上腺素 (NE) 指标家族,nLightG (绿色) 和nLightR (红色).
- 评估这些指标在报告内源性NE释放时的表现,以提高灵敏度,选择性和动力学.
主要方法:
- 基于G蛋白结合受体的基因编码指标的开发.
- 在体外,体外和体内评估指标性能,包括灵敏度,连接体选择性和动力学.
- 在小鼠体内进行多站点纤维光度和双光子成像,以监测NE释放.
主要成果:
- 与现有的GRABNE指标相比,nLightG和nLightR指标显示了检测内源NE释放的提高灵敏度,配体选择性和动力学.
- 通过使用nLightG.实现了光遗传学唤起的NE释放在和海马的同时监测.
- 双光子成像揭示了海马体背部CA1区域的运动和奖励相关的NE过渡体.
结论:
- 新开发的敏感NE指标 (nLightG和nLightR) 显著提高了体内神经调节器成像的能力.
- 这些工具为全面调查北上腺素系统在大脑功能和行为中的作用提供了强大的手段.
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