两个组成的 CroRS 系统作为细胞包膜恒温的主调节器,在细菌病原体 Enterococcus faecalisalis 中赋予抗菌素耐受性
Francesca O Todd Rose1,2, Rachel L Darnell1,2, Sali M Morris3
1Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
Molecular microbiology
|July 20, 2023
概括
在Enterococcus faecalis中,抗菌素耐受性涉及CroRS系统,该系统调节细胞外的完整性. 这个系统将异oprenoid生物合成和呼吸联系起来,以生存抗生素暴露.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 细菌病原体的产生
背景情况:
- 耐药性先于耐药性,对细菌的生存至关重要.
- 两个组成的 CroRS 系统调节了对抗微生物药物的耐受性,例如Enterococcus faecalis.alis 中的 teixobactin.
研究的目的:
- 阐明由CroRS.介导的提克索巴克诱导的抗微生物耐受性背后的分子机制.
- 为了识别CroRS (CroRS regulon) 调节的基因,以应对teixobactin.
主要方法:
- 对野生型和croRS突变的Enterococcus faecalis菌株进行了RNA测序 (RNA-seq).
- 实验进化被用来识别影响耐受性的遗传因素.
- 分析CroRS regulon及其对细胞外生物合成的影响.
主要成果:
- 鉴定了一种132基因的CroRS regulon,CroRS在对teixobactin的反应中对主要细胞包膜组件进行上调.
- 在抗微生物应激期间,CroRS似乎协调了细胞外的完整性.
- 参与去甲基menaquinone合成的HppS的切断,在croRS删除菌株中挽救了耐受性,突出了异oprenoid生物合成的作用.
结论:
- CroRS 作为 Enterococcus faecalis. 细胞外生物发生的主调节剂.
- CroRS作为一个门卫,将异oprenoid生物合成和呼吸联系起来,以确保抗菌素耐受性.
- 了解这种途径对于开发对抗细菌感染的策略至关重要.
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