未成熟的血小板分数随时间的稳定性以及EDTA和引用的全血样本之间的比较
Marc Michel Noel1, Guillaume Feugray2,3, Fiston Kasonga1
1CHU Rouen, Vascular Hemostasis Unit, Rouen, France.
Journal of clinical laboratory analysis
|July 21, 2023
概括
未成熟血小板 (IP) 的测量在EDTA样本中稳定至24小时,在酸盐样本中稳定至6小时. 这项研究支持不成熟血小板分数 (IPF) 分析在诊断血小板缺血时的临床实用性.
科学领域:
- 血液学 血液学 血液学
- 临床病理学 临床病理学
- 诊断研究研究 诊断研究
背景情况:
- 未成熟血小板 (IP) 是年轻的,活跃的血小板,对于诊断血小板缺血至关重要.
- 了解IP的变异性和稳定性对于可靠的临床应用至关重要.
研究的目的:
- 为了比较酸盐与EDTA抗凝剂样本中的不成熟血小板 (IP) 变异性.
- 为了确定IP测量的稳定性在两种样本类型的时间.
主要方法:
- 分析了56名患者,其中28名患者患有血栓塞缩症.
- 使用Sysmex XN-9000 (阻抗计和度计) 测量了血小板数和不成熟的血小板分数 (IPF).
- IPF和绝对不成熟血小板计数 (A-IPC) 通过光方法确定.
主要成果:
- 在EDTA (7.74±6.68%) 和酸盐 (8.45±7.37%) 样本之间没有发现IPF的显著差异.
- 测量IPF显示稳定性在EDTA中长达24小时,在酸盐样本中长达6小时.
- 布兰德-阿尔特曼分析证实,这两种抗凝剂在指定的时间范围内都具有稳定性.
结论:
- 在EDTA中IP测量可靠性高达24小时,在酸盐样本中可靠性高达6小时.
- 这些发现支持IPF分析的潜在临床实用性.
- 需要进一步标准化,以充分定义IPF在临床实践中的作用.
相关概念视频
01:20Complexometric EDTA Titration Curves
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EDTA titration curves determine the free metal ion concentration. The titration curve represents the change in concentration of free metal ions (p function) as a function of the volume of EDTA added. This curve consists of three regions: before, at, and after equivalence points. Excess free metal ions are present before the equivalence point. Equal concentrations of metal ions and EDTA are present at the equivalence point. After the equivalence point, excess EDTA exists. This means slight...
979
01:18Effects of EDTA on End-Point Detection Methods
307
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
307