PIF3被MAPK酸化以调节植物免疫力
Yan Zhao1,2, Xiaojuan Zheng1,3, Xiaojuan Zhang1
1State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
阿拉比多普西斯PIF3通过与MPK3/6.3相互作用来负面调节植物免疫力. 由MPK3/6对PIF3的酸化对于抑制植物防御基因和增加对病原体的敏感性至关重要.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物免疫力 植物免疫力
- 植物病原体相互作用
背景情况:
- 模式触发免疫 (PTI) 是由感知病原体相关分子模式 (PAMPs) 的模式识别受体启动的.
- 线素激活蛋白激酶 (MAPK) 是 PTI 中的关键信号组成部分.
- 了解PTI的规范对于提高作物弹性至关重要.
研究的目的:
- 为了调查Arabidopsis thaliana PIF3在植物防御反应中的作用.
- 阐明PIF3调节模式触发免疫力的机制.
- 在PTI期间确定PIF3的交互合作伙伴和监管修改.
主要方法:
- 酵母两混合测试检测蛋白质与蛋白质相互作用.
- 在体外激酶测试以确认酸化.
- 质谱学和位点定向的突变发生,以确定酸化位点.
- 用Pseudomonas注射器DC3000进行细菌感染测定.
- 定量实时PCR测量防御基因表达.
主要成果:
- 阿拉比多普西斯 PIF3 负面调节植物防御基因表达和对Pseudomonas syringae DC3000的抗性.
- PAMPs触发了PIF3.3的酸化过程.
- 在SP基因残留物中,PIF3与MPK3 / 6相互作用并被MPK3 / 6化.
- 一种模仿素的PIF3变体 (PIF36D) 显示出对P. syringae的易感性增加和防御基因表达的减少.
结论:
- PIF3作为植物免疫的负调节剂.
- 通过MPK3/6介导的PIF3酸化对于其在抑制植物防御中的作用至关重要.
- 在PIF3中酸化特定的SP基因残留物对于调节植物对细菌病原体的免疫力至关重要.
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