Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Fourier-plane spatial frequency modulation imaging: enhanced resolution imaging with a single, ultrashort pulse.

Applied optics·2026
Same author

A double-blind, controlled trial of circadian effective light therapy in patients with Parkinson's disease.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics·2025
Same author

Misspecified Cramér-Rao lower bound with Poisson statistics and its application in localization microscopy.

Optics letters·2025
Same author

Real-time single-element-detection structured illumination optical metrology for laser powder bed fusion.

Optics express·2025
Same author

Invited Session II: Visual Prosthetics: The Neuralink Implant as a visual prosthesis.

Journal of vision·2025
Same author

Agile acousto-optic delay line for fast impulsive stimulated Raman imaging.

Optics letters·2025
Same journal

Long-term stabilization of intensity-difference squeezing from four-wave mixing in rubidium vapor.

Optics express·2026
Same journal

Robust 3D topography measurement of large-range high-aspect-ratio structures based on dual-domain statistical filtering in SD-OCT.

Optics express·2026
Same journal

Broadband transmissive terahertz metasurface for simultaneous quad-mode OAM multiplexing.

Optics express·2026
Same journal

Leveraging two-dimensional materials for high-sensitivity optical sensors: quasi-bound states in the continuum within hybrid metasurfaces.

Optics express·2026
Same journal

Resolution investigation for dual-spherical-wave optical scanning holographic microscopy: methods and performance.

Optics express·2026
Same journal

Robustness of parallel subnetwork-filtered diffractive deep neural networks.

Optics express·2026
查看所有相关文章

相关实验视频

Updated: Jul 22, 2025

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

9.9K

一次性空间频率调制用于成像.

Seth Cottrell, John Czerski, Dan Adams

    Optics express
    |July 21, 2023
    PubMed
    概括
    此摘要是机器生成的。

    我们开发了用于成像的单拍空间频率调制 (SPIFI),消除了对时间变化的调制的需求. 这一创新使得通过一次拍摄同时捕捉所有空间频率,从而实现更快的成像.

    更多相关视频

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    25.3K
    Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis
    10:57

    Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

    Published on: February 1, 2022

    3.1K

    相关实验视频

    Last Updated: Jul 22, 2025

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    9.9K
    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    25.3K
    Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis
    10:57

    Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

    Published on: February 1, 2022

    3.1K

    科学领域:

    • 光学和成像科学科学 光学和成像科学
    • 频谱技术 频谱技术的使用方法

    背景情况:

    • 传统的空间频率调制成像 (SPIFI) 依赖于激发束的时间变化的调制.
    • 这种时间调制限制了成像速度,并且可以复杂地实现.

    研究的目的:

    • 为了引入一种新的单射SPIFI技术.
    • 为了使SPIFI在各种成像应用中实现更快,更简单的实现.

    主要方法:

    • 在光学系统的整个空间带宽中同时实现空间频率调制.
    • 开发了一种用于一次性数据采集的方法,消除了对时间扫描的需求.

    主要成果:

    • 首次成功演示了单次射击SPIFI操作.
    • 在单个曝光中实现了所有空间频率信息的同时获取.

    结论:

    • 单拍SPIFI代表了成像技术的重大进步.
    • 这种方法有可能大大提高成像速度和减少实验复杂性.