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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Updated: Jul 22, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
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使用结构化照明对任意的复杂值对象进行相位检索.

A V Kuzmenko, O M Butok

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    此摘要是机器生成的。

    本研究引入了一种使用两个编码衍射模式 (CDP) 的复杂值对象的相检索新方法. 这种技术允许准确的对象重建与抗噪声和最小的代.

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    科学领域:

    • 光学和光子学 在光学和光子学.
    • 图像重建 图像的重建
    • 计算成像技术的成像

    背景情况:

    • 阶段检索对于从强度测量中重建物体至关重要.
    • 非凸的配方在解决相位检索问题方面存在挑战.
    • 价值复杂的物体需要专门的重建技术.

    研究的目的:

    • 提出一种用于复杂值对象的相检索的新方法.
    • 用支持约束来解决阶段检索问题的非凸式表述.
    • 为了证明两种编码衍射模式 (CDP) 对于对象重建的充分性.

    主要方法:

    • 使用两个随机的二进制振幅面具 (直接和反向) 来生成CDPs.
    • 应用修改后的减少错误或混合输入输出算法来掩盖+CDP对.
    • 从Fresnel或Fraunhofer衍射平面中获得的CDP重建物体.

    主要成果:

    • 两个CDP足以重建具有复杂价值的对象,直到一个全局阶段.
    • 该方法实现了耐噪声和高精度的重建.
    • 对于各种对象类型,有效的重建被证明具有超样本比率≥2.

    结论:

    • 拟议的方法为复杂值对象的相检索提供了一个强大的解决方案.
    • 它适用于不同类型的连贯辐射.
    • 该技术需要少量代才能成功重建.