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基于远端安装的SPAD阵列的手持式广场光终身成像系统

Andrew B Matheson, Ahmet T Erdogan, Charlotte Hopkinson

|July 21, 2023

概括

一个新的手持式光终身成像 (FLIM) 系统使用远端单光子雪崩二极管 (SPAD) 阵列用于ex vivo应用. 这种创新设计能够实现高率成像,并有可能用于体内生物医学应用.

科学领域:

生物医学光学生物医学光学
仪器化仪器化仪器化
显微镜的使用方法

背景情况:

光终身成像 (FLIM) 是一种强大的生物和生物医学分析技术.
现有的FLIM系统通常依赖光纤来传输信号,限制了小型化和手持功能.
需要紧的,便携式FLIM系统用于ex vivo和潜在的in vivo应用.

研究的目的:

为了演示一种新型的手持FLIM系统,其中包含一个远距离安装的单光子雪崩二极管 (SPAD) 阵列.
评估系统是否适用于生物和生物医学环境中的ex vivo应用.
评估开发小型芯片在尖端FLIM内镜系统的潜力.

主要方法:

开发了一种手持FLIM系统,在远端设置了一个<2mm2 128×120 SPAD阵列.
通过>1米长的有线接口与紧型头部 (∼4.5cm3) 操作系统.
该系统的应用用于成像绵羊脏组织和植物叶子,并录制FLIM视频.

主要成果:

成功演示了手持FLIM系统与远距离安装的SPAD阵列.
实现的率超过1Hz,使FLIM视频采集成为可能.
展示了不同组织组成之间的对比成像,以及健康和应激植物叶子之间的对比成像.

结论:

开发的手持FLIM系统是一个重大进步,它是第一款配备远端SPAD阵列的手持FLIM系统.
该系统对体外分析有前途,是迈向微型体内FLIM内镜的关键一步.
紧的设计和高率为手持生物医学成像打开了新的可能性.

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