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基于同步光子辐射的微型计算机断层扫描用于三维增长分析Aspergillus niger颗粒

Henri Müller¹, Charlotte Deffur¹, Stefan Schmideder¹

¹School of Life Sciences Weihenstephan, Chair of Process Systems Engineering, Technical University of Munich, Freising, Germany.

Biotechnology and bioengineering

|July 21, 2023

概括

研究人员使用同步龙X射线微图学分析了来自Aspergillus niger的19,940颗真菌颗粒. 这种方法首次允许详细的3D监测丝状真菌生长和微观形态.

科学领域:

生物技术是生物技术.
微生物学微生物学
生物工艺工程生物工艺工程

背景情况:

丝状真菌对于生产生物技术化合物至关重要.
菌类形态显著影响生产力.
现有的分析真菌宏观形态的方法不足以进行详细的微观形态研究.

研究的目的:

克服实验室微型计算机断层扫描 (μ-CT) 在分析真菌微型学方面的局限性.
开发一种高通量方法来追踪浸泡的真菌颗粒随时间的3D微形态演变.
建立一个全面的形态数据库,以了解真菌生长.

主要方法:

基于同步射线辐射的X射线微图的应用.
分析了来自Aspergillus niger的19940个单独的真菌颗粒.
在48小时的浸水批量种植过程中,在26个点采样.

主要成果:

详细的3D微观形态特性 (子数量,密度,状结构) 确定了每个颗粒.
这项研究首次实现了水下真菌颗粒生长的高分辨率3D监测.
创建了一个大规模的形态数据库.

结论:

同步机μ-CT为真菌微观形态学和生长动态提供了前所未有的洞察力.
生成的数据库可以用于先进的建模和对状真菌种植的理解.
这种方法显著推进了真菌生物过程的分析.

关键词:

3D图像分析分析 3D图像分析黑色阿斯珀吉勒斯 (Aspergillus niger) 是一个计算机断层扫描 (CT) 是一种计算机断层扫描.有丝状真菌的真菌.形态学形态学形态学颗粒的生长成长颗粒的生长.

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