炭醇还原酶:发现,生物合成中的作用和在天然产品合成中的应用
Anshul Rajput1, Tanaya Manna1, Syed Masood Husain1
1Department of Biological and Synthetic Chemistry, Centre of Biomedical Research, SGPGIMS Campus, Raebareli Road, Lucknow 226014, India. smhusain@cbmr.res.in.
Natural product reports
|July 21, 2023
概括
短链脱酶/还原酶 (SDRs) 具有人醇还原酶活性,在天然产品生物合成中至关重要. 这些酶,包括MdpC,能够合成复杂的分子,如亚毒素,并提供应用在化学酶合成.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 自然产品生物合成 自然产品生物合成
背景情况:
- 短链脱酶/减少酶 (SDRs) 是多功能酶,可催化区域和立体选择性减少.
- 在Aspergillus nidulans中发现MdpC的醇还原酶活性,揭示了它在单双丁生物合成中的作用以及对 (R) - 二甲烯的获取.
研究的目的:
- 综合审查过去十年中识别和特征化的人类醇还原酶.
- 总结一下这些酶在天然产品生物合成中的作用.
- 讨论它们在化学酶合成中的应用.
主要方法:
- 关于SDRs和anthrol降解酶的研究的文献综述.
- 对酶表征数据和生物合成途径的分析.
- 报告的化学酶合成应用的汇编.
主要成果:
- 识别了多种与MdpC相关的酶,这些酶参与了阿弗拉托克辛,克拉多富尔文,新沙托林,阿格尼斯和双氨基的生物合成.
- 这些酶的特征是作为利用NADPH进行 antraquinone 减少的anthrol 减少酶.
- 证明它们在合成各种化合物中的实用性,包括flavoskyrins和 chiral cycloketones.
结论:
- 炭醇还原酶是一种重要的SDR类,在微生物的二次代谢中发挥着不同的作用.
- 这些酶代表了复杂的天然产品及其类型的化学酶合成的宝贵工具.
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