从欧米克到哺乳动物细胞工厂发展中的细胞机制
Mojtaba Samoudi1, Helen O Masson2, Chih-Chung Kuo2
1Department of Pediatrics, University of California, San Diego, La Jolla, California, USA.
Current opinion in chemical engineering
|July 21, 2023
概括
哺乳动物细胞工厂可以改造,以改善生物制药生产. 奥米克和系统生物学方法提供了强大的策略来克服低蛋白质产量和提高细胞工厂效率.
科学领域:
- 生物技术是生物技术.
- 细胞生物学 细胞生物学
- 生物加工是一种生物加工.
背景情况:
- 哺乳动物细胞对于生产具有类似人类修饰的复杂生物治疗药物至关重要.
- 低蛋白质产量限制了哺乳动物细胞工厂对某些产品的应用.
- 传统的细胞工程方法与细胞路径和修改的复杂性作斗争.
研究的目的:
- 讨论提高哺乳动物细胞工厂中的蛋白质生产率的策略 (CHO,HEK293).
- 探索全基因组查和多基因组学指导细胞工程的潜力和局限性.
- 突出系统生物学在理解细胞机制中的作用,以实现有效的工程.
主要方法:
- 对生物制药生产的细胞工程策略的审查.
- 讨论全基因组查和多omics方法.
- 在细胞工厂优化中探索系统生物学应用.
主要成果:
- 基于Omics和系统的方法为理想的细胞工厂表型提供了宝贵的见解.
- 这些先进的方法可以指导细胞工程提高蛋白质生产率.
- 通过系统生物学了解细胞机制,有助于开发有效的工程策略.
结论:
- 整合omics和系统生物学是克服哺乳动物细胞工厂生产率局限性的关键.
- 全基因组查和多组学为理性细胞工程提供了强大的工具.
- 系统生物学完善了我们对细胞过程的理解,使生物制药生产更有效.
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