在C.I.C.中, 热带通过通过自细胞下调瘤细胞内在的PD-1受体来促进CRC
Junxing Qu1,2,3, Qianyu Chen1,3, Ziqian Bing1,3
1The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing, China.
Journal of Cancer
|July 21, 2023
概括
热带菌通过降低瘤细胞编程细胞死亡1 (PD-1) 的调节来促进结肠癌的进展,这种调节是由增加的自介导的. 这一发现提供了关于结直肠癌 (CRC) 免疫疗法耐药性的见解.
科学领域:
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
背景情况:
- 编程细胞死亡1 (PD-1) 是一种免疫检查点分子,参与免疫耐受性和瘤免疫逃生.
- 瘤细胞内在的PD-1在黑色素瘤,肝癌和肺癌等癌症中发挥了多种作用,但其在结肠癌中的功能尚不清楚.
- 以前的研究表明,Candida tropicalis可促进结直肠癌 (CRC) 的生长和抗化学药性.
研究的目的:
- 研究瘤细胞内在PD-1在结肠癌进展中的作用.
- 确定Candida tropicalis是否通过PD-1影响CRC进展和免疫疗法耐药性.
- 阐明CRC中Candida tropicalis介导的PD-1调节的基本机制.
主要方法:
- 评估了Candida tropicalis对结肠癌细胞生长在体外和体内移植的作用.
- 在结肠癌细胞系中研究PD-1表达和操纵PD-1水平 (敲击/过度表达).
- 利用对PD-1的阻断抗体和自抑制剂来研究机制.
主要成果:
- 高度的Candida tropicalis促进了CRC瘤的生长.
- 结肠癌细胞表达PD-1;PD-1的淘汰增强了SW480细胞的活力,而过度表达则减少了它.
- 热带菌下调瘤细胞内在PD-1表达,促进CRC进展,部分通过增强的自.
- 阻断PD-1促进了瘤的生长,而不依赖于适应性免疫.
结论:
- 在结直肠癌中,PD-1 作为瘤抑制剂起作用.
- 热带菌通过增强的自增强促进CRC进展,通过降低瘤细胞内在PD-1的调节.
- 这种机制可能解释了某些免疫疗法在某些癌症患者中的无效性.
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