相关实验视频

Updated: Jul 22, 2025

Induction and Analysis of Epithelial to Mesenchymal Transition

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

在SKOV3细胞中TGFß1诱导的EMT中表达双特异性酸酶

Sabire Güler¹, Abdullah Yalçın²

¹Department of Histology and Embryology, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa, Turkey.

Turkish journal of medical sciences

|July 21, 2023

概括

转化生长因子β1 (TGFβ1) 在卵巢癌细胞中诱导表皮-介质细胞转换 (EMT),改变双特异性酸酶 (DUSP) 表达,增加基因激活蛋白激酶 (MAPK) 活性.

科学领域:

分子生物学分子生物学
癌症研究癌症研究
细胞生物学细胞生物学

背景情况:

卵巢腺癌的进展涉及表皮-介质细胞过渡 (EMT).
双特异性酸酶 (DUSP) 在细胞信号通路中发挥作用.
转化生长因子β1 (TGFβ1) 是EMT的一个关键诱导因子.

研究的目的:

为了研究在卵巢腺癌细胞中TGFβ1诱导的上皮-介质细胞过渡 (EMT) 期间双特异性酸酶 (DUSP) 的表达特征.
在EMT的背景下分析TGFβ1对MAPK信号通路的影响.

主要方法:

卵巢腺癌SKOV3细胞被TGFβ1治疗以诱导EMT.
使用实时qPCR,西斑和EMT标志物的免疫光学来验证EMT.
DUSP mRNA表达和MAPK酸化水平分别通过实时qPCR和西部斑点进行评估.

主要成果:

TGFβ1治疗降低了E-cadherin和增加了牛和牛的表达,证实了EMT.
在TGFβ1诱导后,ERK1/2和p38 MAPK的酸化升高.
特定的DUSP成员 (DUSP2,6,8,10,13) 减少,而其他成员 (DUSP7,16,18,21,27) 增加.

结论:

在SKOV3细胞中TGFβ1诱导的EMT与增加的MAPK活性有关.
在卵巢癌细胞中,EMT显著改变了各种DUSP家族成员在卵巢癌细胞中的表达模式.

关键词:

双重特异性的酸酶.这就是MAPKK的意思.在SKOV3中,SKOV3是什么?在TGFß1上.表皮细胞-介质细胞过渡.卵巢癌 (卵巢癌) 是一种卵巢癌.

更多相关视频

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Published on: August 3, 2018

相关概念视频

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...