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在食道状细胞癌中,Circ_0058063调节细胞活力和增殖
Yixuan Yang1, Bing Zhu2, Zhaofeng Ning3
1Department of Health Care, The First Affiliated Hospital of Xiamen University, Xiamen, China.
Journal of biochemical and molecular toxicology
|July 21, 2023
概括
循环RNA hsa_circ_0058063 促进食道状细胞癌 (ESCC) 通过海绵miR-4319和升级THBS1.1. 这一发现为ESCC治疗提供了潜在的向疗法.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 胃肠病学 胃肠病学
背景情况:
- 食道状细胞癌 (ESCC) 由于预后不佳,因此具有重大临床挑战.
- 驱动ESCC进展的精确分子机制尚未完全阐明.
- 循环RNA hsa_circ_0058063 (circ_0058063) 已涉及促进ESCC,但其监管作用需要进一步调查.
研究的目的:
- 研究食道状细胞癌 (ESCC) 中circ_0058063的调节机制.
- 探索ESCC中circ_0058063,microRNA-4319 (miR-4319) 和血栓蛋白-1 (THBS1) 之间的关系.
- 评估ESCC中针对circ_0058063的治疗潜力.
主要方法:
- 定量实时聚合酶连锁反应 (qRT-PCR) 和西部斑分析,以评估circ_0058063,miR-4319和THBS1.1的表达水平.
- 细胞功能测试包括CCK8,Edu,流细胞计和Transwell测试,以评估细胞活力,增殖,亡,迁移和入侵.
- 双露西法酶记者测定以确认miR-4319和circ_0058063或THBS1.1之间的相互作用.
- 使用小鼠模型进行体内实验,以验证circ_0058063对瘤生长的影响.
主要成果:
- 与正常对照组相比,ESCC组织和细胞中的Circ_0058063和THBS1水平显著上调,而miR-4319水平则下调.
- 耗尽circ_0058063抑制了ESCC细胞的增殖,迁移和入侵,并促进了细胞亡.
- 证实miR-4319通过向THBS1.1来抑制ESCC的进展.
- Circ_0058063作为miR-4319的分子海绵,从而调节THBS1的表达,促进ESCC的发展.
- 在体内减弱了circ_0058063的瘤生长.
结论:
- Circ_0058063促进ESCC的发展,通过通过海绵miR-4319.3通过THBS1表达升级调节THBS1表达.
- 该circ_0058063/miR-4319/THBS1轴代表了食道状细胞癌的潜在治疗标.
- 向circ_0058063可能为ESCC治疗提供一种新的策略.
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