相关实验视频
Updated: Jul 22, 2025
05:44
Performing In Vivo and Ex Vivo Electrical Impedance Myography in Rodents
Published on: June 8, 2022
3.0K
结合电肌学和拉曼光谱学:光学EMG.
James J P Alix1,2, Maria Plesia1, Pamela J Shaw1,2
1Sheffield Institute for Translational Neuroscience, University of Sheffield, Sheffield, UK.
Muscle & nerve
|July 21, 2023
概括
光学EMG在一个针头中结合了电生理学和拉曼光谱学,为神经肌肉疾病提供了一个新的生物标志物. 这项技术成功地在小鼠模型中区分了健康和患病的肌肉.
科学领域:
- 神经肌肉疾病诊断神经肌肉疾病诊断
- 生物分子光谱学
- 医疗器械 医疗器械 医疗器械
背景情况:
- 电肌图 (EMG) 对于诊断神经肌肉疾病至关重要,但缺乏分子洞察力.
- 拉曼光谱为组织提供特定的分子指纹,成为潜在的生物标志物.
- 综合方法可以提高肌肉疾病的诊断能力.
研究的目的:
- 引入和验证"光学EMG",一种结合EMG和拉曼光谱的新技术.
- 评估使用单针同时获取电生理学和分子数据的可行性.
- 评估光学EMG作为生物标记物的潜力,在肌缩侧面硬化症 (ALS) 的小鼠模型中.
主要方法:
- 开发了一种能够收集电生理学和拉曼光谱数据的专用针.
- 对SOD1G93A转基因小鼠 (ALS模型) 和非转基因对照进行了体内实验.
- 记录了复合肌动力潜能 (CMAP),自发EMG活动,以及来自胃肌的拉曼光谱.
主要成果:
- 光学EMG成功记录了与标准EMG针相比的电生理学数据.
- 在SOD1G93A和对照小鼠之间观察到CMAP振幅的显著差异.
- 拉曼光谱检测显示了两组之间肌肉组织中明显的分子组成差异,仅在转基因小鼠中检测到自发的EMG活性.
结论:
- 光学EMG从单针插入中提供标准电生理学和分子拉曼数据.
- 这种综合方法显示出作为神经肌肉疾病诊断的新生物标志物的潜力.
- 该技术成功地在相关的动物模型中区分了健康和患病的肌肉.
更多相关视频
相关概念视频
01:26Raman Spectroscopy Instrumentation: Overview
455
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
455
01:20Raman Spectroscopy: Overview
458
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
458