结合的细胞外矩阵线索与机械应变诱导了对肌肉发生的差异性影响in vitro
Alex H P Chan1,2,3, Ishita Jain1,2,3, Beu P Oropeza1,2,3
1Stanford Cardiovascular Institute, Stanford, CA, USA. ngantina@stanford.edu.
Biomaterials science
|July 21, 2023
概括
这项研究揭示了细胞外矩阵 (ECM) 和机械应变如何影响肌肉再生. 生物仿真组织芯片表明,将ECM与机械应变相结合,可以协同增强肌管形成和用于肌肉修复的基因表达.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 细胞生物学 细胞生物学
背景情况:
- 骨肌肉再生对于治疗肌肉损失和缩至关重要.
- 目前的方法缺乏对影响肌肉发生的微环境因素的理解.
- 生物仿真平台是需要研究复杂的细胞过程.
研究的目的:
- 研究细胞外基质 (ECM) 和机械应变对肌肉发育的联合影响.
- 开发一种仿生组织芯片,用于研究肌肉再生.
- 分析肌肉发育过程中的转录和翻译变化.
主要方法:
- 开发了可伸缩组织芯片,使用原I,纤维菌素和拉米林的组合.
- 应用静态和动态 (无轴) 机械应变 (5%和15%).
- 进行了转录基因组分析 (全基因组) 和翻译评估.
主要成果:
- ECM的组成显著影响了神经管的形成;拉米尼促进了长度,纤维素促进了宽度.
- 组合ECM对肌管发育表现出复杂的,非直观的影响.
- 机械应变协同增强了肌管宽度,并提高了肌重链基因的调节.
结论:
- ECM组成和机械应变是肌肉发生的关键调节者.
- 生物仿真组织芯片提供了一个平台来剖析微环境对肌肉再生的影响.
- 了解这些因素可以为骨肌肉修复的策略提供信息.
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