[长跨基因非编码RNA 01121通过激活EMT过程促进前列腺癌细胞的转移]
Jie Wu1, Li-Xin Hua2, Fa-de Liu1
1Department of Urology, Jiangning Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210000, China.
Zhonghua nan ke xue = National journal of andrology
|July 21, 2023
概括
长跨基因非编码RNA 01121 (LINC01121) 在前列腺癌 (PCa) 中过度表达. 减少LINC01121通过影响表皮细胞-介质细胞过渡 (EMT) 来抑制PCa细胞的增殖,迁移和入侵.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 遗传学 是一个遗传学.
背景情况:
- 前列腺癌 (PCa) 是一个重大的健康问题.
- 长非编码RNA (lncRNAs) 在包括癌症在内的各种生物过程中起着至关重要的作用.
- 在PCa中LINC01121的作用仍然在很大程度上未被探索.
研究的目的:
- 为了研究PCa.中LINC01121的表达水平.
- 为了阐明PCa细胞系中LINC01121的生物功能.
- 探索LINC01121影响PCa进展的潜在机制,特别关注上皮层-介质酶过渡 (EMT).
主要方法:
- 实时定量聚合酶链反应 (qRT-PCR) 用于评估LINC01121表达.
- 使用小干扰RNA (siRNA) 来抑制LINC01121的表达.
- 进行了细胞增殖,克隆,迁移和入侵试验 (CCK8,细胞克隆,Transwell).
- 西方斑点分析被用来确定EMT相关蛋白的表达.
主要成果:
- 与正常前列腺细胞相比,LINC01121表达在PCa细胞系中显著上调.
- 抑制LINC01121显著抑制了PCa细胞的增殖,殖民地形成,迁移和入侵.
- LINC01121 knockdown导致N-cadherin和vimentin的表达减少,E-cadherin的表达增加,表明EMT的抑制.
结论:
- 在前列腺癌中,LINC01121是致癌的.
- 过度表达LINC01121促进PCa细胞的增殖,迁移和入侵.
- LINC01121可能通过激活PCa中的EMT过程来发挥其致癌作用.
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