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miRNA 511_5p 是眼睛毒素菌的潜在生物标志物

Geraldo Magela de Faria Júnior¹, Laurie Sayuri Kumano¹, Isabela Bronchtein Gomes¹

¹Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), 5416 Brigadeiro Faria Lima Avenue, São José do Rio Preto, SP, 15090-000, Brazil; Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.

Transactions of the Royal Society of Tropical Medicine and Hygiene

|July 21, 2023

概括

微RNA 511_5p在患有活性眼部毒素质症 (OT) 的患者中显示出高度. 这一发现表明它有可能成为这种炎症性眼病的诊断生物标志物.

科学领域:

眼科医生眼科眼科
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

眼部毒素菌 (OT) 是一种由*Toxoplasma gondii*感染引起的炎症性眼部疾病.
通过促炎性细胞因子激活巨细胞驱动OT中的炎症过程.
微RNA,包括miRNA511,在炎症期间调节巨细胞激活起作用.

研究的目的:

在被诊断患有眼部毒素菌症的患者中评估miRNA 511_5p的表达.
为了确定miRNA 511_5p水平是否可以区分活跃的OT,治愈的OT和健康的个体.

主要方法:

一组361名患者被分为四组,根据眼部病变和*Toxoplasma gondii*血清学.
进行了包括血清学在内的临床和实验室评估,以确认OT诊断.
进行了RNA提取,cDNA合成和血液等离子体中miRNA 511_5p水平的定量分析.

主要成果:

患有活跃眼部病变和阳性*Toxoplasma gondii*血清学 (G1) 的患者表现出显著更高的miRNA 511_5p (平均22.34) 的血度.
在G1中增加的miRNA511_5p水平与那些有痕的人 (G2:4.65),没有病变/痕但是血清阳性的人 (G3:8.91) 和血清阴性对照人 (G4:3.52) 相比.
统计分析显示,小组之间的miRNA 511_5p水平存在非常显著的差异 (p<0.0001).

结论:

这项研究表明,miRNA 511_5p在活跃的眼球毒中显著上调.
这些发现表明,miRNA 511_5p作为诊断OT的潜在生物标志物具有相当大的前景.
进一步的研究可能会验证miRNA 511_5p作为眼部毒素菌的非侵入性诊断工具.

关键词:

这种毒性淋巴细胞 (Toxoplasma gondii) 是微型RNA 511_5pp 是一个微型RNA.眼睛中的毒素菌.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...