ER局部化Shr3是一种选择性的共同翻译折叠伴侣,对于氨基酸透酶生物发生是必要的
Ioanna Myronidi1, Andreas Ring1, Fei Wu2
1Department of Molecular Biosciences, The Wenner-Gren Institute, SciLifeLab, Stockholm University, Stockholm, Sweden.
Shr3是一种伴侣蛋白,有助于将氨基酸浸透酶 (AAP) 插入到内细胞网膜 (ER) 中. 它与新生的AAP链相互作用,促进它们的正确折叠和功能.
科学领域:
- 细胞生物学 细胞生物学
- 贩卖蛋白质 贩卖蛋白质 是一个问题.
- 膜生物学 膜生物学
背景情况:
- 具有多个膜跨越段 (MS) 的蛋白质以协同翻译的方式插入到真核内 плазма网膜 (ER) 膜中.
- Shr3是一种ER膜局部化的伴侣,对于含有12个MS的氨基酸浸透酶 (AAP) 的功能表达至关重要.
研究的目的:
- 为了研究Shr3和氨基酸浸透酶 (AAP) 之间的体内伴侣基质相互作用.
- 阐明Shr3促进多膜跨越蛋白质的插入和折叠的机制.
主要方法:
- 对Shr3蛋白的全面扫描突变发生和删除分析.
- 经过修改的分裂-乌比奎丁试验,以探测体内伴侣-基质相互作用.
- 对AAP和非Shr3基质糖载体与嵌套的C端截断相互作用的分析.
主要成果:
- 与糖载体不同,Shr3选择性地与AAP截断相互作用,表明基质特异性.
- 随着MS细分数的增加,Shr3-AAP相互作用会加强,但当所有12个MS都存在时,它们会显著减弱.
- 相互作用是结构性的,涉及Shr3的膜和光域,而不是特定于序列的.
结论:
- Shr3充当了支架,在翻译过程中参与了AAP的新生N端链.
- 这种相互作用促进了AAP的共同翻译折叠和膜插入.
- Shr3的机制涉及结构识别,支持复杂的多膜跨越蛋白质的插入.
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