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Preparation of DNA-crosslinked Polyacrylamide Hydrogels
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在可切换生物电催化和可控释放负荷的表面上,以刺激反应的DNA为基础的水凝
Michael Fadeev1, Gilad Davidson-Rozenfeld1, Zhenzhen Li1
1The Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
ACS applied materials & interfaces
|July 21, 2023
概括
在电极上装有酶的DNA水凝为受控生物电催化提供可调节的刚性. 这使可切换的葡萄糖氧化酶功能和触发胰岛素释放成为可能,进步了智能生物传感器技术.
科学领域:
- 生物材料科学 生物材料科学
- 生物电化学 生物电化学
- DNA DNA 纳米技术 纳米技术
背景情况:
- 响应刺激的水凝可以动态控制材料的特性.
- 含有酶的水凝对于生物传感和药物输送应用至关重要.
- 基于DNA的水凝提供了精确的结构控制和生物相容性.
研究的目的:
- 为可切换生物电催化功能开发含酶,对刺激有反应的DNA水凝.
- 为了证明可调节的生物活性对水凝刚度的触发控制.
- 为了实现控制葡萄糖度,从修改后的电极触发潜在的胰岛素释放.
主要方法:
- 在电极表面上组装含氧化糖酶 (GOx) 的DNA水凝.
- 使用pH响应的i-动机和K+-离子/皇冠以太响应的G-四重复的DNA结构进行交联.
- 研究由pH,K+离子和皇冠引发的水凝刚度变化.
- 使用电子介质器或集成矩阵演示潜在触发的,血糖控制的胰岛素释放.
主要成果:
- 成功组装了GOx载荷的,具有可调节刚性的刺激响应DNA水凝.
- 通过改变水凝硬度,证明了GOx可切换的生物电催化活性.
- 从水凝修饰的电极中释放激发的胰岛素,以应对葡萄糖度和电位.
- 展示了两种不同的水凝系统,分别对pH和K+离子做出反应.
结论:
- 具有可调节刚性的酶载DNA水凝为可切换生物电催化剂提供了一个平台.
- 开发的系统可以精确控制酶活性和药物释放.
- 这种方法对先进的生物传感器,药物输送系统和生物电子设备具有前景.
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