在Hydra中,卡斯帕酶介导的LPS传感和烧信号传递
Shouwen Chen1, Shuxin Li1, Hao Chen1
1State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology, Shanghai 200237, China.
Science advances
|July 21, 2023
概括
研究人员在Hydra中发现了一种由脂聚糖 (LPS) 诱导的热灭菌信号通路,涉及气体皮质胺 (GSDM) 和炎症性囊. 这种古老的机制突出显示了对从Cnidarians到哺乳动物的细菌感染的保护免疫防御.
科学领域:
- 这是天生的免疫力.
- 细胞微生物学 细胞微生物学
- 进化免疫学的进化免疫学
背景情况:
- 炎症性卡斯帕斯和气体皮质胺 (GSDM) 介导的灭对于哺乳动物的抗感染免疫是至关重要的.
- 在早期元动物中感知LPS和启动热的精确机制在很大程度上仍未被探索.
研究的目的:
- 为了阐明脂多糖 (LPS) 诱导的Cnidarians,特别是在Hydra. pyroptosis信号通路.
- 为了确定关键的分子参与者,包括卡斯帕和GSDM蛋白,参与这个古老的免疫反应.
主要方法:
- 在Hydra.中的GSDM (HyGSDME) 和caspase (HyCaspA,HyCARD2) 蛋白质的识别和功能性表征.
- 研究由细胞质LPS引发的卡斯巴依赖性火灭菌级联.
- 在Hydra的息肉中对HyGSDME进行局部化研究,并评估其在抗菌防御中的作用.
主要成果:
- 在Hydra中发现了一种功能性的GSDM蛋白,HyGSDME,在感知细胞质LPS时调解卡斯巴酶依赖性热.
- 发现一种新的炎症性酶,HyCaspA,通过其疏水性N端域感知细胞质LPS,从而导致自我激活.
- HyCaspA激活了HyCARD2,这反过来又触发了HyGSDME介导的灭,表明了跨物种保存的古老信号级联.
结论:
- 一个古老的LPS诱导和GSDM-gated pyroptosis信号级联已经在Hydra中得到解决.
- 这些发现表明,炎症性体感知细胞质LPS以启动GSDM导入的热致死,代表了从Cnidarians到哺乳动物的保存免疫防御机制.
- HyGSDME在保护Hydra免受表面入侵细菌的影响方面发挥着重要作用,这凸显了这一途径的进化意义.
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