在母的生殖道,卵白和胚胎附件中的阿迪波金表达
Ophélie Bernardi1, Guillaume Bourdon2, Anthony Estienne2
1SYSAAF French Poultry and Aquaculture Breeders Technical Center, Research for Agriculture, Food and Environment Institute Val de Loire Center, F-37380 Nouzilly, France; French National Centre for Scientific Research, French Horse and Riding Institute, Research for Agriculture, Food and Environment Institute, Tours University, Physiology of Reproduction and Behavior Unit, F-37380 Nouzilly, France.
Poultry science
|July 21, 2023
概括
阿迪波金及其受体存在于的卵白,卵管和胚胎组织中. 这些代谢激素可以作为生物标志物来增强胚胎发育.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 鸟类生理学 鸟类生理学
背景情况:
- 脂肪组织分泌的阿迪波金素会影响哺乳动物的生殖功能.
- 化学素是一种阿迪波金,在鸟类蛋膜中丰富,在胚胎膜中表达.
- 其他阿迪波金在鸟类繁殖和胚胎发育中的作用不太清楚.
研究的目的:
- 为了研究在鸟类生殖道和胚胎附录中的阿迪波涅克丁,维斯法丁,阿佩林和阿迪波林及其受体的表达.
- 为了比较活跃和回归卵管中的阿迪波金表达.
- 为了确定胚胎发育过程中这些阿迪波金的存在和动态.
主要方法:
- 西方斑点分析分析
- RT-qPCRR 是一个人.
- 免疫组织化学 免疫组织化学
- 来自活跃和退化的产母母的卵管分析.
- 收集不同发育阶段的胚胎液 (羊水和羊液).
主要成果:
- 阿迪波涅丁,维斯法丁,阿佩林和阿迪波林及其受体在卵管和蛋专辑中表达.
- 这些阿迪波金和受体的表达在回归卵管中显著减少.
- 在的胚胎发育过程中,维斯法丁和阿迪波涅丁水平在羊水和亚兰托液中发生变化.
结论:
- 阿迪波金及其受体是鸟类生殖系统和胚胎发育的组成部分.
- 阿迪波金表达模式表明,它在调节生殖功能和支持胚胎生长方面起着至关重要的作用.
- 阿迪波基因显示出作为改善胚胎发育的生物标志物的潜力.
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