通过超高性能液体染色学-高分辨率质谱学对人类眼中的未知物质进行非有针对性的分析和初步鉴定:试点研究试点研究:

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Updated: Jul 22, 2025

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

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Pablo Dualde¹, Pablo Miralles¹, Cristina Peris-Martínez²

¹Foundation for the Promotion of Health and Biomedical Research in the Valencian Region (FISABIO-Public Health), Valencia, Spain.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

|July 21, 2023

概括

使用UHPLC-HRMS的新方法在人类眼中确定了58种化合物,包括代谢产物和药品. 这种经过验证的方法为发现新生物标志物和暴露物质提供了高精度.

科学领域:

分析化学分析化学
生物化学生物化学
药理学药理学是指药理学的学科.

背景情况:

人的眼分析对于理解生理和病理状态至关重要.
识别眼中未知的化合物可以揭示新的生物标志物和暴露指标.
需要先进的分析技术来进行全面的撕裂分析.

研究的目的:

开发和验证一种新的UHPLC-HRMS方法,用于识别人类眼中的未知化合物.
建立一个用于眼样本分析的自动化工作流.
评估该方法在生物标志物和暴露发现方面的潜力.

主要方法:

超高性能液态染色学-高分辨率质谱学 (UHPLC-HRMS) 具有智能数据采集 (AcquireX DS-dd-MS2).
使用Compound DiscovererTM 3.2软件进行自动化数据处理.
在正和负电离模式下分析了四个人类眼样本.

主要成果:

58种物质的初步鉴定,包括氨基酸,激素,代谢物和药品.
确定的化合物的度范围从0.1到9000 ng/mL.
方法验证证实了29个标准中的28个 (96.6%的准确率).

结论:

开发的UHPLC-HRMS方法对于识别人类眼中未知的化合物是有效的.
这种方法有助于发现新的代谢物和潜在的生物标志物.
这种选工具可以通过撕裂分析帮助识别环境暴露,如污染物.

关键词:

人类生物监测人类生物监测人类的眼是人类的眼.眼科医生眼科眼科在UHPLC-HRMS中使用.未知物质未知物质没有目标的分析分析.

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