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CNT-FET用于通过固定的Cytochrome c通过敏感的过氧化生物传感

Elaheh Mozayan¹, Hossain-Ali Rafiee-Pour¹, Foad Ghasemi²

¹Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, Iran.

Archives of biochemistry and biophysics

|July 21, 2023

概括

一种新型的生物传感器利用碳纳米管场效应晶体管 (cMWCNTs/FETs) 上固定的细胞染色体c (Cyt c) 可实时检测过氧化 (H2O2). 这种敏感和选择性的平台为潜在的诊断应用提供了快速的H2O2测量.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
生物传感器是一种生物传感器.

背景情况:

过氧化 (H2O2) 在生物系统中起着双重作用,对细胞过程至关重要,但在高度下有害,与大肠炎和全身性红斑狼等疾病有关.
准确和实时监测H2O2水平对于理解其生理和病理作用以及早期疾病诊断至关重要.

研究的目的:

设计和开发一种新的生物传感器,用于敏感和选择性地实时检测过氧化 (H2O2).
在各种实验条件下研究开发的生物传感器的性能特征.

主要方法:

一个场效应晶体管 (FET) 生物传感器的制造,通过将细胞染色体c (Cyt c) 固定在碳纳米管 (CNT) 薄膜 (cMWCNTs/FET) 上.
实时监控Cyt c/cMWCNTs/FET的输出电流变化,以应对H2O2的增加.
通过改变Cytc和cMWCNTs度和pH水平来优化生物传感器性能.

主要成果:

在Cyt c/cMWCNTs/FET生物传感器检测到H2O2时,输出电流出现下降,表明传感成功.
在H2O2检测中实现了高度敏感的线性响应范围,从10.0 fM到1.0 nM.
显示低检测极限 (9.13 fM) 和快速响应时间 (约1.0 秒),对常见干扰物质具有很好的选择性.

结论:

开发的Cyt c/cMWCNTs/FET生物传感器是高度敏感,选择性和快速实时检测过氧化的有希望的平台.
这种生物传感器具有各种生物医学应用的潜力,包括疾病诊断和氧化应激监测.

关键词:

细胞染色体c 在检测检测检测检测检测场效应晶体管的领域效应晶体管.过氧化是一种过氧化.多面墙碳纳米管是多面墙碳纳米管.实时响应实时响应

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