Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Enzymes02:34

Enzymes

81.9K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
81.9K
Single-pass Transmembrane Proteins01:25

Single-pass Transmembrane Proteins

5.1K
Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
5.1K
Introduction to Enzymes01:22

Introduction to Enzymes

18.7K
The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that...
18.7K
The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

2.5K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
2.5K
Membrane Proteins01:30

Membrane Proteins

20.8K
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
20.8K
Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

5.8K
Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Environmentally persistent free radicals lead to selective inhibition of CYP1 monooxygenase activities, and increased production of reactive oxygen species by reaction uncoupling.

Frontiers in public health·2025
Same author

Environmentally persistent free radicals stimulate CYP2E1-mediated generation of reactive oxygen species at the expense of substrate metabolism.

Drug metabolism and disposition: the biological fate of chemicals·2025
Same author

Identification of the N-terminal residues responsible for the differential microdomain localization of CYP1A1 and CYP1A2.

The Journal of biological chemistry·2024
Same author

Environmentally Persistent Free Radicals stimulate CYP2E1-mediated generation of reactive oxygen species at the expense of substrate metabolism.

Drug metabolism and disposition: the biological fate of chemicals·2024
Same author

Improving healthcare professionals' ultrasound-guided peripheral vascular access ability utilizing self-assembled ultrasound phantoms: A prospective, observational quality improvement project.

The journal of vascular access·2024
Same author

The Influence of Lipid Microdomain Heterogeneity on Protein-Protein Interactions: Proteomic Analysis of Co-Immunoprecipitated Binding Partners of P450 1A2 and P450 3A in Rat Liver Microsomes.

Drug metabolism and disposition: the biological fate of chemicals·2023

相关实验视频

Updated: Jul 22, 2025

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
12:15

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

Published on: February 21, 2019

7.5K

CYP1酶的功能性表征:复杂的形成,膜局部化和功能.

J Patrick Connick1, James R Reed1, George F Cawley1

  • 1Department of Pharmacology and the Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center -, New Orleans, LA 70112, USA.

Journal of inorganic biochemistry
|July 21, 2023
PubMed
概括

细胞染色体P450 (CYP) 蛋白质CYP1A1,CYP1A2和CYP1B1表现出不同的四级结构和细胞定位,影响它们作为多酶系统的功能. 这些差异影响蛋白质-蛋白质相互作用和基质代谢,挑战了它们作为简单单质酶的观点.

关键词:
在CYP1A1中.在CYP1A2中.在CYP1B1B1中.细胞染色体P450的使用.P450与P450的相互作用蛋白质微域本地化.

更多相关视频

Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins
10:21

Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins

Published on: June 20, 2019

24.1K
In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
10:05

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

Published on: August 13, 2012

11.4K

相关实验视频

Last Updated: Jul 22, 2025

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
12:15

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

Published on: February 21, 2019

7.5K
Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins
10:21

Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins

Published on: June 20, 2019

24.1K
In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
10:05

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

Published on: August 13, 2012

11.4K

科学领域:

  • 生物化学 生化学
  • 分子生物学分子生物学
  • 酶学 是一种酶学.

背景情况:

  • 细胞染色体P450 (CYP) 酶CYP1A1,CYP1A2和CYP1B1共享序列相似性,但表现出功能上的差异.
  • 了解它们的四级结构和细胞定位对于阐明它们在多酶系统中的作用至关重要.

研究的目的:

  • 描述蛋白质-蛋白质复合体的形成,脂质微域本地化,以及CYP1A1,CYP1A2和CYP1B1.1的功能.
  • 研究这些特性如何影响它们在NADPH-cytochrome P450减少酶 (POR) 的背景下酶活性.

主要方法:

  • 生物发光共振能量转移 (BRET) 用于检测同质和异质复合物的形成.
  • 酶活性被测量为不同NADPH-cytochrome P450减少酶 (POR) 度的函数.
  • 评估了脂质微域本地化,以了解膜分区.

主要成果:

  • 与CYP1B1.1不同的是,CYP1A1和CYP1A2形成了同质复合体.
  • CYP1A2与POR表现出西格形动力学,这表明复合体形成会影响功能,而CYP1A1和CYP1B1显示出过度反应.
  • 观察到明显的异构复合体形成 (CYP1B1/CYP1A1),但不是在CYP1A2和其他之间.
  • CYP1A2和CYP1B1局限于有序的膜微域,而CYP1A1则在无序的区域中发现.

结论:

  • 尽管序列相似,但CYP1蛋白在四级结构,复杂形成和膜局部化方面表现出显著的差异.
  • 这些结构和局部化变异影响它们的酶功能和在内质网膜内的相互作用.
  • 应该将CYP1蛋白质视为复杂的多酶系统的组成部分,而不是孤立的单体.