中红外光谱学用于大规模表型化牛乳总成分和免疫球蛋白度
A Goi1, A Costa2, G Visentin2
1Department of Agronomy, Food, Natural resources, Animals and Environment, University of Padova, 35020 Legnaro (PD), Italy.
Journal of dairy science
|July 21, 2023
概括
中红外光谱学 (MIRS) 可以准确预测牛乳中的免疫球蛋白G (IgG) 和蛋白质含量. 这种快速,具有成本效益的方法有助于评估新生儿反动物免疫力和工业应用的乳酪质量.
科学领域:
- 兽医免疫学和诊断 兽医免疫学和诊断
- 食动物的营养和健康
- 在动物科学中的光谱分析.
背景情况:
- 通过胆乳传递的被动免疫转移对于新生儿的反动物的生存至关重要.
- 准确评估乳酪蛋白免疫球蛋白G (IgG) 和成分至关重要,但传统上是昂贵和耗时的.
- 需要快速,可扩展的方法来评估乳牛奶的质量,以获得最佳的小牛健康和工业用途.
研究的目的:
- 评估中红外光谱学 (MIRS) 的预测能力,以确定牛乳中的IgG度和总成分.
- 评估MIRS作为一种快速且具有成本效益的替代方法,用于大规模结母乳分析的黄金标准方法.
- 为了确定MIRS预测值的分辨能力,用于分类乳母质量.
主要方法:
- 分析了714个意大利霍尔斯坦牛乳液样本,这些样本是在分娩后6小时内采集的.
- 在水中稀释样品后,使用基板光谱仪进行中红外光谱学 (MIRS) 分析.
- 通过将光谱数据与IgG,总Ig,蛋白质,脂肪和乳糖的参考测量相关联,开发了预测模型.
主要成果:
- 在交叉验证中,MIRS在IgG度和成分特征的预测性表现良好至优异 (R2CV ≥0.92).
- 外部验证显示,IgG (R2V=0.84),蛋白质 (R2V=0.89) 和脂肪 (R2V=0.74) 的预测能力很有希望.
- 根据预测的IgG和蛋白质含量,MIRS有效地区分了低质量和高质量的乳头,准确度很高.
结论:
- 中红外光谱 (MIRS) 是一种快速,具有成本效益的工具,用于量化牛乳中的IgG,蛋白质和乳糖.
- MIRS促进了大规模的乳酪质量查,支持育种者和农民在管理和选择策略.
- 这些发现支持MIRS在常规实验室测试中的实施,用于结母乳分析,在食品和制药行业有潜在的应用.
关键词:
动物健康动物健康一只小牛犊子大奶粉是什么? 大奶粉是什么? 大奶粉是什么?乳牛牛奶牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛免疫的被动转移 免疫的被动转移更多相关视频
07:19Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn Zea mays As an Exemplar
Published on: August 6, 2018
20.0K
08:42Methods for Comparing Nutrients in Beebread Made by Africanized and European Honey Bees and the Effects on Hemolymph Protein Titers
Published on: March 17, 2015
10.7K
相关概念视频
IR and UV–Vis Spectroscopy of Carboxylic Acids
4.3K
In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency,...
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency,...
4.3K
Infrared (IR) Spectroscopy: Overview
1.9K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
Different compounds display unique properties due to their...
1.9K
