单层离心法作为一种用于辅助生殖的公牛精液中细菌减少的方法
Aleksandar Cojkic1, Ingrid Hansson2, Anders Johannisson3
1Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, 75007, Sweden. aleksandar.cojkic@slu.se.
Veterinary research communications
|July 21, 2023
概括
单层离心法有效地从公牛精液中去除细菌,显著提高精子的运动性和减少DNA碎片化. 高密度和低密度的方法都提高了精子质量,使SLC成为适合细菌消毒的技术.
科学领域:
- 兽医科学 兽医科学 兽医科学
- 生殖生物学 生殖生物学
- 微生物学 微生物学
背景情况:
- 牛精液中的细菌污染会对精子质量产生负面影响.
- 有效的细菌去除对于优化人工授精成功至关重要.
研究的目的:
- 评估高密度和低密度单层离心法 (SLC) 来从公牛精液中去除细菌.
- 评估SLC治疗对公牛精子质量参数的影响.
主要方法:
- 使用了细菌量化 (CFU/mL) 和识别 (MALDI-TOF MS) 的方法.
- 使用计算机辅助精子分析 (CASA) 评估了精子运动性.
- 精子染色素完整性通过精子染色素结构试验 (SCSA) 进行评估.
主要成果:
- 高密度和低密度SLC都显著降低了细菌的数量 (p < 0.001).
- 高密度SLC对Bacillus和Proteus spp.特别有效.
- 治疗后观察到CASA参数的显著改善和DNA碎片化的减少.
结论:
- 使用高密度或低密度合体的单层离心法 (SLC) 是减少公牛精液中的细菌的可行方法.
- SLC治疗对关键的精子质量指标产生积极影响,包括运动性和DNA完整性.
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