相关实验视频

Updated: Jul 22, 2025

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

系统性审查和元分析:在胚胎囊阶段进行前植入基因检测是否可以提高活产率?

Lorraine S Kasaven^1,2, Diana Marcus^3,4, Efstathios Theodorou⁵

¹Department of Cancer and Surgery, Imperial College London, Exhibition Rd, South Kensington, London, SW7 2BX, UK. lk226@doctors.org.uk.

Journal of assisted reproduction and genetics

|July 21, 2023

概括

在胚胎囊阶段进行前植入基因检测 (PGT-A) 增加了每次胚胎移植的活产和持续怀孕率. 此外,与传统的形态学评估相比,这种方法还可以显著降低流产率.

科学领域:

生殖医学生殖医学
遗传学遗传学是一个
在体外受精过程中受精.

背景情况:

对于用于体外受精 (IVF) 的胚胎的传统评估依赖于形态评估.
胚胎前植入基因测试 (PGT-A) 旨在确定胚胎中的染色体异常.
在活检后,PGT-A通常在胚囊阶段进行.

研究的目的:

为了确定PGT-A在囊胚阶段是否改善与标准形态评估相比,活产和正在进行的怀孕率.
评估PGT-A对流产率的影响.

主要方法:

在PubMed,EMBASE和Cochrane数据库 (2000年3月 - 2022年3月) 中进行了系统的文献搜索.
包括的研究比较了IVF结果,使用在囊胚阶段的综合染色体查 (CCS) 与传统的形态评估.
来自6项随机对照试验 (RCT) 和10项队列研究的数据被汇总起来进行分析.

主要成果:

在RCT (RR 1.09) 和队列研究 (RR 1.50) 中,PGT-A在活产率和每胚胎移植的持续怀孕率的复合结果中显示出显著的好处.
通过CCS识别的欧体胚胎显示出更高的植入率 (RCT:RR 1.20;队列:RR 1.69).
每个临床怀孕中流产率在CCS实施后显著降低 (RCT:RR 0.73;队列:RR 0.48).

结论:

在囊胚阶段基于综合性染色体查的PGT-A改善了每次胚胎移植的活产和持续怀孕率.
与单独的形态评估相比,PGT-A显著降低了流产率.
由于研究数量有限和方法变化,进一步的研究是有必要的.

关键词:

胚胎囊中的胚胎细胞.不孕不育不孕不育不孕不育活生生的出生率是多少进行元分析分析.持续的怀孕率持续的怀孕率植入前遗传检测,检测出无细胞积分症.系统性审查系统性审查

更多相关视频

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis

Published on: September 7, 2021

Human Blastocyst Biopsy and Vitrification

Human Blastocyst Biopsy and Vitrification

Published on: July 26, 2019

相关概念视频

Nondisjunction

Nondisjunction

Nondisjunction is the failure of homologous chromosomes or sister chromatids to separate correctly and move to the opposite poles of the cells. This produces daughter cells with abnormal chromosome numbers. Nondisjunction is common during anaphase I or anaphase II of meiosis. Mutations in synaptonemal complex proteins that attach homologous chromosomes increase the chances of nondisjunction in anaphase I of meiosis I. In contrast, mutations in topoisomerases and condensins that hold...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

Teratogenicity

Teratogenicity

The ability of a drug to produce structural deformations and functional abnormalities in the developing embryo or the fetus is called teratogenicity, and the drug producing this effect is known as a teratogen. Teratogenic effects include stillbirth, miscarriage, intrauterine growth restriction, and neurocognitive delay. A teratogen may affect the embryo at different stages of development, which is important in determining the type and extent of the damage. During blastocyst formation, the early...

Cleavage and Blastulation

Cleavage and Blastulation

After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.