Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA Splicing01:32

RNA Splicing

56.5K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.5K
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

15.3K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
15.3K
Alternative RNA Splicing02:18

Alternative RNA Splicing

21.4K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.4K
X-linked Traits01:19

X-linked Traits

55.0K
In most mammalian species, females have two X sex chromosomes and males have an X and Y. As a result, mutations on the X chromosome in females may be masked by the presence of a normal allele on the second X. In contrast, a mutation on the X chromosome in males more often causes observable biological defects, as there is no normal X to compensate. Trait variations arising from mutations on the X chromosome are called “X-linked”.
55.0K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

5.3K
5.3K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

2.7K
2.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Decoupling Thermodynamic Dilemma in the Crystallization of Cs<sub>3</sub>BiSbBr<sub>9</sub> Bimetallic Perovskites for Photocatalytic HBr Splitting.

Inorganic chemistry·2026
Same author

RelA-mediated (p)ppGpp accumulation links glucose starvation to peptidoglycan synthesis arrest and spherical morphogenesis via PBP2x and FtsX in <i>Streptococcus suis</i>.

Journal of bacteriology·2026
Same author

Global Research Trends of Diabetes Mellitus and Metabolic Reprogramming: A Bibliometric and Visualization Analysis.

Journal of visualized experiments : JoVE·2026
Same author

The interactions and combined effects of benzo[<i>a</i>]pyrene with other environmental pollutants.

iScience·2026
Same author

Multimodal analytical strategy for revealing the related compounds of color variation in Salvia miltiorrhiza after sweating.

Journal of pharmaceutical and biomedical analysis·2026
Same author

An Environmentally Tolerant 5A Hydrogel with Photothermal Effect for Frostbite Treatment.

Gels (Basel, Switzerland)·2026

相关实验视频

Updated: Jul 22, 2025

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
11:19

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

15.0K

使用拼接定量特征位点绘制对复杂特征有贡献的内部保留事件的映射.

Siyuan Wang1, Hongyu Wu1, Yongyan Zhao1,2

  • 1Zhejiang Provincial Key Laboratory of Crop Genetic Resources, Institute of Crop Science, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 300058, China.

Plant methods
|July 21, 2023
PubMed
概括

替代拼接影响作物产量. 拼接定量特征位点 (sQTL) 被确定为精密棉花育种的新型遗传资源,有助于提高产量和质量.

关键词:
另一种拼接 (AS)棉花 (Gossypium hirsutum L.) 是一种棉花.纤维的收益率是可以获得的.内部保留 (IR) 是指分离定量特征位置 (sQTL)

更多相关视频

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.5K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

2.8K

相关实验视频

Last Updated: Jul 22, 2025

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
11:19

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

15.0K
ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.5K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

2.8K

科学领域:

  • 植物遗传学 植物遗传学
  • 分子生物学分子生物学
  • 农业学是一种农业学.

背景情况:

  • 替代拼接 (AS) 的mRNA对于转录组多样性至关重要,并调节植物生长和应激反应.
  • 了解作物种群中的AS变化,可以了解复杂的农学特征.
  • 对作物中AS事件的全基因组关联研究 (GWAS) 是有限的.

研究的目的:

  • 开发一条管道,用于识别与高地棉花复杂特征相关的候选AS事件.
  • 研究AS的作用,特别是内部保留 (IR) 在调节棉纤维产量和质量的作用.

主要方法:

  • 在高地棉花种群 (279行) 中使用卵子转录组数据对内子保留 (IR) 的全基因组表征.
  • 拼接定量特征位点 (sQTL) 分析以确定与AS事件相关的遗传变异.
  • 将sQTL分析与表达量性特征定位 (eQTL) 和GWAS定位集成.

主要成果:

  • 共有2295个涉及1607个基因的sQTL被确定,其中14.25%是cis-regulatory.
  • 53%的cis-sGenes是由cis-sQTL和cis-eQTL共同调节的.
  • 32个cis-QTL与GWAS位点共同定位纤维产量和质量特征,表明它们的调节作用.
  • 像GhLRRK1和GhGC1这样的基因中的差异性IR率与毛皮百分比 (LP) 相相关.

结论:

  • 替代拼接显著影响作物产量和质量特征.
  • 功能性sQTLs代表了在棉花中精密繁殖的宝贵遗传资源.
  • 识别的AS事件和相关基因为改善棉纤维特征提供了潜力.