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转录组景观揭示了来自猪皮肤的多能皮肤纤维细胞原始体的生殖线潜力

Wen-Xiang Liu^1,2, Chun-Xiao Li¹, Xin-Xiang Xie¹

¹College of Life Sciences, Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, Qingdao Agricultural University, Qingdao, 266109, China.

Cellular and molecular life sciences : CMLS

|July 22, 2023

概括

皮肤衍生干细胞 (SDSC) 可以分化为生殖细胞,为不孕症治疗提供了新的希望. 这项研究揭示了它们的起源以及它们发展成原始生殖细胞样细胞 (PGCLCs) 的复杂过程.

科学领域:

干细胞生物学干细胞生物学
生殖医学是一种生殖医学.
再生医学是一种再生医学.

背景情况:

在全球范围内,不孕症影响约15%的夫妇.
现有的辅助生殖技术对于精或卵细胞异常无效.
皮肤衍生干细胞 (SDSCs) 分化为原始生殖细胞样细胞 (PGCLCs) 是一个有前途的治疗途径.

研究的目的:

为了研究猪SDSCs的细胞起源.
为了阐明SDSC分化为PGCLCs期间的转录形状变化.
探索SDSC衍生的PGCLCs在不孕症治疗中的潜力.

主要方法:

使用单细胞RNA测序来分析猪SDSCs.
实验室差异化协议被用于从SDSCs生成PGCLC.
对转录调节的分析,包括基质子修饰和信号通路.

主要成果:

猪SDSCs主要来源于由EGF/bFGF调节的多能皮肤纤维细胞原始体 (MDFPs).
通过复杂的转录调节,SDSCs表现出多能性和有效分化为PGCLCs.
由SDSC衍生的PGCLC与人类原始生殖细胞共享保存特征,其增殖由MAPK信号通路介导.

结论:

这项研究提供了关于干细胞在体外分化成生殖细胞的新见解.
这些发现突出了SDSC衍生的PGCLCs对精和缺陷卵细胞的潜在治疗应用.
了解SDSCs的细胞起源和分化动态对于在生殖健康方面推进再生医学至关重要.

关键词:

在 MAPK 信号通道中.多能皮肤纤维细胞的祖先.原始的生殖细胞类细胞.单细胞转录组是一个单细胞转录组.皮肤衍生干细胞来源于皮肤.

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