同时测定水溶性除草剂使用水友互动液体染色学-质谱学
Daisuke Watanabe1, Shuhei Sonoda2, Hikoto Ohta2
1National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa City, Chiba, 277-0882, Japan. watanabe.daisuke@nrips.go.jp.
Forensic toxicology
|July 22, 2023
概括
这项研究提出了一种快速的方法来分析水溶性除草剂,如草甘和酸,而无需衍生. 该技术在中毒和作物损害案件中的法医应用中是有效的.
科学领域:
- 分析化学 分析化学
- 法医毒理学 法医毒理学
背景情况:
- 水溶性除草剂如草甘 (Glyp),酸 (Gluf),酸 (PQ) 和二酸 (DQ) 需要专门的,耗时的和昂贵的分析.
- 对于这些化合物,一般的毒理学选方法是不够的.
研究的目的:
- 为Glyp,Gluf,PQ和DQ开发一种简单,快速和同时的分析方法.
- 在分析中避免衍生和离子配对试剂.
主要方法:
- 用水友互动液态色谱 (HILIC) 来进行分析物分离.
- 为了检测,采用了并行质谱法 (MS/MS).
- 该方法应用于植物和生物样本.
主要成果:
- 对于所有分析品,都实现了优异的分离和峰值形状.
- 在植物样本 (枯的叶子) 中,专门使用简单的提取方法检测出除草剂.
- 在生物样本中验证的定量分析显示,Glyp和Gluf的量化极限为0.2μg/mL,PQ和DQ的量化极限为1ng/mL.
结论:
- 开发的HILIC-MS/MS方法适用于水溶性除草剂的同时分析.
- 该方法显示出足够的性能用于实际的法医应用,包括中毒调查和作物损害评估.
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