相关实验视频
Updated: Jul 22, 2025

06:34
The Caco-2 Cell Bioassay for Measurement of Food Iron Bioavailability
Published on: April 28, 2022
4.8K
常见豆的内在标签用2H2O,以使蛋白质可消化的估计
Karina Pfrimer1,2, Roberto Botelho Ferraz Branco3, Tom Preston4
1Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
Isotopes in environmental and health studies
|July 22, 2023
概括
通过使用 (2H) 成功创建了内在标记的普通豆种子. 在充分开花阶段施加2H2O,可以为人类消化研究的豆进行最佳丰富.
科学领域:
- 农业科学 农业科学
- 生物化学 生物化学
- 人类营养人类营养
背景情况:
- 稳定同位素标记植物蛋白为评估人类蛋白质吸收效率提供了一种新方法.
- (2H) 纳入常见豆 (Phaseolus vulgaris L.) 蛋白质的实质性标签正在探索中.
- 该研究调查了将2H纳入种子氨基酸的最佳现象学阶段.
研究的目的:
- 为了确定种子成熟的最有效阶段,将稳定的同位素 (2H) 纳入常见豆氨基酸中.
- 为了确定2H2O应用的最佳时间和数量,用于普通豆种子的内在标签.
- 为了验证2H丰富普通豆种子对人类营养研究的适用性.
主要方法:
- 常见豆种植在中,并在播种后50天接受氧化 (2H2O) 应用.
- 随后的2H2O应用是在初次应用后的3,6,9和12天间隔进行的.
- 应用2H2O的时间与种子发育的不同现象学阶段相关.
主要成果:
- 在所有治疗组中,蛋白质结合氨基酸中的丰富度都达到了500ppm以上.
- 应用2H2O在最初应用后的6,9和12天,与完全开花阶段一致,产生了最高的丰富度.
- 这些发现表明,普通豆种子的内在标签成功使用.
结论:
- 所有经过测试的2H2O应用策略都在用丰富豆种子方面被证明是有效的.
- 在完全开花阶段2H2O应用的时间对于最大限度地将纳入种子氨基酸至关重要.
- 内在2H标记的普通豆种子适合开发试验餐,以评估人类的消化和必需氨基酸吸收.
关键词:
氨基酸是氨基酸的组成部分.气-2 气-2 是一种气.同位素生态学 同位素生态学这种植物是Phaseolus vulgaris L.消化能力 消化能力食物 食物 食物 食物植物蛋白质是一种植物蛋白质.稳定同位素追踪技术是稳定的同位素追踪技术.更多相关视频
07:34Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide
Published on: May 12, 2023
1.1K
10:16Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling
Published on: January 16, 2014
22.1K
相关概念视频
Labeling DNA Probes
8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
Protein Digestion
104.2K
Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of polypeptide chains. After polypeptide chains are broken into individual amino acids by a series of digestive enzymes, the amino acids are transported to the liver via the bloodstream to produce energy.
104.2K
¹H NMR of Labile Protons: Deuterium (²H) Substitution
926
This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
926