在第一季度尿样中测量24个甲酸代谢物:MIREC研究
Tye E Arbuckle1, Éric Gaudreau2, Susan MacPherson1
1Environmental Health Sciences and Research Bureau, Health Canada, Ottawa, ON, K1A 0K9, Canada.
Chemosphere
|July 22, 2023
概括
这项研究开发了一种新的方法来测量24个甲酸代谢物在孕妇中,改善了对某些代谢物的检测,并为环境化学风险评估确定了新的代谢物.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 毒理学 毒理学 毒理学
背景情况:
- 酸盐是常见的环境化学物质,被检测为尿液代谢产物.
- 之前的研究测量了有限数量的甲酸代谢产物.
- 目前正在确定新的甲酸代谢产物.
研究的目的:
- 开发一种分析方法来量化24种甲酸代谢物,包括新发现的代谢物.
- 为了提高对先前测量的甲酸代谢物的敏感性.
- 分析大量孕妇参与者的甲酸代谢物水平.
主要方法:
- 使用超高性能液态染色学与双重质谱学 (UPLC-MS/MS) 相结合.
- 开发了一种高分辨率的四极飞行时间 (QToF) 系统,用于同位素和干扰区分.
- 从环境化学品母婴研究 (MIREC) 研究中分析了大约1800个生物银行尿样.
主要成果:
- 成功开发了一种方法来测量从10个原始甲酸盐中24个甲酸盐代谢物.
- 对某些甲酸盐复杂异构混合物的染色学解释存在观察到的挑战.
- 罕见地检测到单环-基甲酸盐 (MCHP),单基甲酸盐 (MCHpP),单基甲酸盐 (MiDP) 和单基甲酸盐 (MnOP).
- 改善了单甲基甲酸盐 (MMP) 的检测.
- 由于异构复杂性,部分代谢物 (MCiOP,MiNP,MCiNP) 被半量化报告.
结论:
- 开发的方法可以在孕妇人群中进行全面的甲酸盐生物监测.
- 精确量化复杂的甲酸盐同位素混合物需要仔细的分析考虑.
- 产生了宝贵的生物监测数据,用于对孕妇的甲酸盐风险评估.
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